导数光度法直接测定血清中痕量锌的研究及应用  

SECOND-DERIVATIVE SPECTROPHOTOMETRIC DETERMINATION OF ZINC WITH MESO-TETRA-(3,5-DIBROMO-4-HYDROXYPHENYL) PORPHYRIN IN SERUM

作　　者：汤坚[1] 李在均 王利平[1] 孙文旦[2] 潘教麦[3] 

机构地区：[1]无锡轻工大学,无锡214036 [2]无锡市第二人民医院 [3]华东师范大学化学系

出　　处：《理化检验（化学分册）》1999年第11期492-493,495,共3页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

摘　　要：建立了一种以锌与meso-四-(3,5-二溴-4-羟基苯基)卟啉显色反应为基础的二阶导数光度法测定血清中锌的方法.在10ml溶液中,0～2μgZn^(2+)符合比耳定律.血清中共存元素不干扰锌的测定,其二阶导数光度法的检出限为1.86ng·ml^(-1),已用于血清中锌的直接测定.结果满意.It is reported in this paper that a method of secondary derivetive spectrophotometric determina-tion of trace amounts of zinc in human blood is proposed. Meso-tetra-(3,5-dibromo-4-hydroxyphenyl) porphyrin is used as chromogenic reagent for Zn(Ⅱ) ion. The determination range is 0-2. 0μg of zinc in 10ml solution and the detection limit is found to be 1. 86ng · ml^(-1). Most of the metal ions in reasonable amounts do not interfere with the determination of zinc in blood serum.

关 键 词：导数光度法 血清 锌 测定 

分 类 号：R446.11[医药卫生—诊断学]