P53基因在电离辐射诱导的非小细胞肺癌细胞死亡中的作用  

The role of P53 gene in radiation-induced death of non-small-cell lung cancer

作　　者：乔俊[1] 马淑梅[2] 李英普[3] 宋志恒[2] 易贺庆[2] 侯吉光[1] 贾立立[2] 谭哲峰 刘晓冬[2] 王铁君[1] 

机构地区：[1]吉林大学第二医院肿瘤放疗科,吉林长春130021 [2]吉林大学公共卫生学院卫生部放射生物学重点实验室,吉林长春130021 [3]吉林大学中日联谊医院骨科,吉林长春130021 [4]吉林省大唐长山电厂职工医院,吉林松原138000

出　　处：《中国实验诊断学》2011年第5期776-779,共4页Chinese Journal of Laboratory Diagnosis

基　　金：国家自然科学基金资助项目(30770649)国家自然科学基金资助项目(30970682)

摘　　要：目的研究p53基因在电离辐射诱导的非小细胞肺癌细胞死亡中的作用。方法选取H1299(p53-/-),H1299-P53,H1299-175H细胞为研究对象。采用流式细胞术检测细胞凋亡率,WESTERN BOLT方法检测相关蛋白表达情况。结果在H1299(P53-/-)细胞中,4GY、8GY照射后细胞凋亡率分别是假照组的68.4%、50%。在H1299-P53细胞中,4GY、8GY照射后细胞凋亡率分别是假照组的1.7倍、8.1倍。在H1299-175H细胞中,4GY8、GY照射后细胞凋亡率分别是假照组的1.5倍、2倍。LC3蛋白表达在H1299呈现剂量依赖性升高,在H1299-175H呈现剂量依赖性降低,在H1299-P53中,OGY表达最高,4 GY表达最低,8 GY表达低于假照组但比4 GY高。AKT表达在H1299细胞呈剂量依赖性降低,H1299-P53在4GY表达量升高而在8GY又降低但仍高于假照组,在H1299-175H细胞中AKT表达呈剂量依赖性降低。MDM2在H1299的表达呈剂量依赖性升高,在H1299-P53细胞中呈现与AKT相同的变化趋势,在H1299-175H细胞中4GY时表达量最高8GY最低。Caspase-3在H1299细胞中随着照射剂量的增加变化不明显,在H1299-P53细胞中呈现剂量依赖性升高,在H1299-175H细胞中,0 GY表达最高,4 GY表达最低,8 GY低于假照组但高于4 GY组。结论 X射线促进P53(-/-)的H1299细胞时发生自噬,而促进H1299-P53及H1299-175H中发生凋亡。Objective To investigate the role of P53 gene in radiation induced cell death of non-small-cell lung cancerMethods Select the H1299（p53-/-）,H1299-P53,H1299-175H cells for the studing.Flow cytometry was used to detect the apoptosis rate,western bolt was used to detect the expression of related autophagy and apoptosis protein.Results When treated with 4 GY、8 GY H1299（P53-/-） manifest the lower apoptosis rate by 68.4%and 50%respectively compared with sham-irradiation group.When treated with 4GY 、8GY H1299-P53 manifest the highter apoptosis rate by 1.7 times and 8.1 times respectively compared with sham-irradiation group .When treated with 4 GY、8 GY H1299-175H manifest the highter apoptosis rate by 1.5 times and 2 times respectively compared with sham-irradiation group.The expression of akt is decreasing in dose dependent manner in H1299,the same in H1299-175H,but in H1299-p53 and,the expression of akt elevated in 4 GY,decreased in 8GY but higher compared with sham-irradiation group.The expression of MDM2 show positive dose-dependent manner in H1299（P53-/-）,the trend of the expression of MDM2 in H1299-P53 show the same tread as expression of akt,but in H1299-175H the expression of MDM2 increased in 4GY,decreased in 8GY.The expression of LC3 show positive dose-dependent manner in H1299（P53-/-）,but show negative dose-dependent manner in H1299-P53 and H1299-175H.The expression of caspase-3 show no clear difference in H1299（P53-/-）,in H1299-P53 the expression of caspase-3 increasing in dose dependent manner,in H1299-175H the expression of caspase-3 increased in 0 GY,decreased in 4 GY.Conclusion The effect of X-ray in H1299（P53-/-）promote autophagy,but promote apoptosis in H1299-P53 and H1299-175H.

关 键 词：P53 H1299 AKT1 MDM2 LC3 Caspase-3 

分 类 号：R734.2[医药卫生—肿瘤]