含HN基因的重组鸡痘病毒抑制骨肉瘤细胞SAOS-2的体外实验研究  

The vitro empirical study about apoptosis of SAOS-2 induced with recombinant Fowl-pox comprised HN gene

作　　者：张治宇[1] 苑福升[1] 李力卓[2] 胡硕[3] 孔大亮[4] 高志学[5] 

机构地区：[1]中国医科大学附属第四医院骨科,辽宁沈阳110031 [2]中国医科大学附属盛京医院骨科 [3]第二军医大学长海医院骨科 [4]吉林大学中日联谊医院 [5]中国医科大学北京顺义医院骨科

出　　处：《中国实验诊断学》2011年第5期816-818,共3页Chinese Journal of Laboratory Diagnosis

摘　　要：目的探讨含HN的重组鸡痘病毒(rFPVHN)对骨肉瘤细胞SAOS-2的抑制效应。方法以rFPVHN感染人骨肉瘤细胞SAOS-2,应用MTT法检测细胞活性,罗丹明123结合FACS测定线粒体跨膜电位,测定肿瘤细胞表面唾液酸含量,底物染色反映检测Caspase-3活性。结果 rFPVHN感染可有效抑制SAOS-2肿瘤细胞,杀伤率达58.9%。感染使SAOS-2肿瘤细胞线粒体膜电位下降,导致SAOS-2肿瘤细胞凋亡。细胞表面唾液酸水平明显下降;Caspase-3活性被激活。结论 rFPVHN可诱导SAOS-2肿瘤细胞凋亡。Objective To study the effection about apoptosis of SAOS-2 induced with recombinant Fowl-pox comprised HN gene.Methods The recombinant Fowl-pox virus infected human osteosarcoma cells SAOS-2,The viability of SAOS-2 cells was determined by MTT staining and the alteration of mitochondrial transmembrane potential was determined by FCM with Rhodamine 123.The activation of caspase-3 Was assayed by substrate color reaction and determined sialic-acid on cells.Results After recombinant Fowl-pox virus infected SAOS-2 cells mortality of cells was increased and viability was decreased significantly respectively.Compared with control cells,mitochondrial transmembrane potential was decreased,sialic-acid level of the cells Was increased,and caspase-3 was activate.Conclusion rFPVHN can induced the apoptosis of SAOS-2 cells.

关 键 词：重组鸡痘病毒 骨肉瘤细胞 细胞凋亡 

分 类 号：R738.1[医药卫生—肿瘤]