白内障晶状体上皮细胞Bcl-2Bax表达的增龄性研究  

Research the relationship between cataract lens epithelial cell and Bcl-2, Bax expression with aging

作　　者：杜敏[1] 田蕊蕊[1] 牛永亮[1] 

机构地区：[1]晋中市第二人民医院眼科,山西晋中030800

出　　处：《中国实用眼科杂志》2011年第6期620-622,共3页Chinese Journal of Practical Ophthalmology

摘　　要：目的探讨随着年龄的增长白内障晶状体上皮细胞Bcl-2、Bax的基因表达与白内障发生的关系，及其在白内障发生发展中的作用机制。方法选取行超声乳化白内障手术的患者91例91只眼，取晶状体前囊膜。根据年龄分为3组，即I组为≤30岁的患者11例，Ⅱ组为31～60岁的患者30例，Ⅲ组为I〉61岁的患者50例。采用链霉菌亲生物素蛋白-过氧化物酶标免疫组织化学法（streptavidin—peroxidase，SP）染色，镜下检测三个不同年龄组白内障患者晶状体上皮细胞Bcl-2基因蛋白与Bax基因蛋白的表达情况。结果I、Ⅱ、Ⅲ组Bcl-2基因表达分别为89％、60％、0．5％，Bax基因表达分别为0、11％、91％。各项指标经统计学分析具有统计学意义（P〈0．05o结论随着年龄的增长白内障晶状体上皮细胞Bcl-2基因表达逐渐降低，Bax基因表达逐渐增高，为在基因水平上治疗白内障提供了理论依据。Objective To evaluate the relationship between cataract lens epithelial cell and Bcl-2, Bax expression with increasing age in patients, and to investigate the role of Bcl-2 and Bax expression in the occurrence and progression of human lens epithelial cells of cataract. Methods Ninety-one patients （91 eyes） who were done phacoemulsification for cataract were selected and took anterior capsule of lens. The cataract patients were divided into three groups according to age： 11 cases of less than 30 years of age in group I, 30 cases of aged 31-60 in group lI, 50 eases more than 61 years of age in group III. The expression of Bcl-2 and Bax in lens epithelial cells of cataract from three different age groups of cataract patient was detected by the immunohistochemical streptavidin-peroxidase （SP） method. Results The Bcl-2 gene expression in group I, II, and III was 89%, 60% and 0.5% respectively, the Bax gene expression was 0, 11% and 91% respectively. The difference had the statistical significance between 3 groups （P 〈0.05）. Conclusions The genes expression of Bcl-2 in human lens epithelial cells of cataract is gradually decreasing with age increasing, while the genes expression of Bax is gradually increasing with age increasing. It provides an important theoretical basis to treat of cataract at the gene level.

关 键 词：白内障晶状体上皮细胞 BCL-2 BAX 免疫组织化学 

分 类 号：R779.66[医药卫生—眼科]