抑制枯否细胞对脓毒症大鼠肝脏微循环的影响  

Changes of liver microcirculation in septic rats after the inhibition of Kupffer cells

作　　者：杜施霖[1] 姚晨玲[1] 栾骁[1] 张亚平[1] 樊帆[1] 薛渊[1] 李鑫[1] 童朝阳[1] 

机构地区：[1]复旦大学附属中山医院急诊科,上海200032

出　　处：《中国急救医学》2011年第6期518-521,共4页Chinese Journal of Critical Care Medicine

基　　金：复旦大学青年启动基金项目（No.1-54）

摘　　要：目的探讨抑制枯否细胞（Kupffer cells，KCs）对脓毒症大鼠肝脏微循环的影响。方法采用盲肠结扎穿孔法（CLP）制备大鼠脓毒症急性肝损伤模型。造模前1d和2d经尾静脉注射三氯化钆（GdCl3）去除KCs。将60只健康SD大鼠随机分为四组，即对照组、假手术组（Sham组）、模型组（CLP组）和GdCl3组（CLP＋GdCl3组）。各组动物于术后24h处死。检测血中丙氨酸氨基转移酶（ALT）、天门冬氨酸氨基转移酶（AST）、内毒素（ET）、内皮素-1（ET-1）及一氧化氮（NO）的水平。提取肝组织RNA，采用RT～PCR方法检测ET—1、eNOS、INOS及HO—1mRNA的表达。结果与对照组比较，脓毒症大鼠血中ALT、AST、ET、ET-1及NO水平显著升高（P〈0．05）；去除KCs后，脓毒症大鼠血中ALT、AST、ET、ET—1及NO水平显著降低（P〈0．05）。脓毒症大鼠肝组织ET-1、eNOS、iNOS及HO-1mRNA表达水平较对照组显著升高（P〈0．05）；去除KCs后，肝组织中iNOS及HO-1mRNA表达水平显著降低（P〈0．05）。结论KCs在脓毒症时肝脏的微循环障碍中起重要作用。Objective To investigate the influence of Kupffer cells （KCs） inhibition on liver microcirculation in the rats with sepsis. Methods The sepsis model of Sprague - Dawley （SD） rat by cecal ligation and puncture （CLP） was used. The KCs were inactivated by gadolinium chloride （ GdCl3 ） at 1 and 2 d before operation via tail vein. Sixty healthy male SD rats were randomly divided into 4 groups：control group, sham operation group （Sham group）, sepsis group （CLP group）and GdCl3 group （CLP ＋ GdC13 group）. All rats were sacrificed at 24 h after operation. The blood was collected to detect the levels of alanine aminotransferase （ALT）, aspartate aminotransferase （AST）, endotoxin （ET）, endothelin - 1 （ ET - 1 ） and nitrogen monoxide （NO）. The liver was harvested to analyze the messenger RNA （mRNA） expressions of ET - 1, eNOS, iNOS and HO - 1 by reverse transcriptase polymerase chain reaction （ RT - PCR）. Results Compared with control group, the levels of ALT, AST, ET, ET - 1 and NO in blood were significantly increased in CLP group （ P 〈 0.05 ）. The increases were suppressed after the inhibition of KCs by GdCI3. Moreover, compared with control group, the mRNA expressions of ET - 1, eNOS, iNOS anti HO - 1 were significantly increased after CLP, This increases in the mRNA expression of iNOS and HO - 1 were attenuated by GdCls （ P 〈 0.05 ）. Conclusion The KCs may play an important role in the disturbance of liver mierocirculation in sepsis.

关 键 词：枯否细胞 脓毒症 微循环障碍 三氯化钆 

分 类 号：R[医药卫生]