间断性缺氧致大鼠认知功能障碍与脑nAChRα4表达的关系  

Relationship between cognition disorders caused by intermittent hypoxia and expression of nicotinic acetylcholine receptor alpha4 in brain in rats

作　　者：陈燕[1] 赵春玲[1] 张春来[1] 徐倩[1] 

机构地区：[1]泸州医学院生理教研室,四川泸州646000

出　　处：《重庆医学》2011年第17期1675-1676,1679,F0002,共4页Chongqing medicine

摘　　要：目的探讨间断性缺氧(IH)致大鼠认知功能障碍与脑烟碱型乙酰胆碱受体α4(nAChRα4)表达的关系。方法将成年雄性Sprague-Dawley(SD)大鼠40只随机分为对照组I、H 1周组I、H 3周组及IH 5周组。采用Morris水迷宫检测认知功能的改变,免疫组化法检测前额叶皮层和海马神经元nAChRα4蛋白的表达。结果定位航行实验中,与对照组及IH 1周组比较,IH5周组大鼠逃避潜伏期明显延长(P<0.05)。空间搜索实验中,与对照组比较,IH 5周组大鼠原平台所在象限停留时间百分率明显减少(P=0.047)。大鼠前额叶皮层和海马神经元nAChRα4蛋白的表达随缺氧时间延长呈下降趋势。结论间断性缺氧致大鼠认知功能障碍可能与前额叶皮层和海马神经元nAChRα4表达降低有关。Objective To explore relationship between cognition disorders caused by intermittent hypoxia and expression of nicotinic acetylcholine receptor alpha4（nAChRα4） in brain in rats.Methods Forty adult male Sprague-Dawley（SD） rats were randomly divided into four groups：control group,IH one week group,IH three weeks group and IH five weeks group.Changes of cognitive function were assessed by Morris Water Maze and protein expression of nAChRα4 in neurons of prefrontal cortex and hippocampus were detected by immunohistochemical staining.Results Compared with control group and IH one week group,the escape latency of rats in IH five weeks group were significantly prolonged（P0.05） in place navigation tests,and the percentage of time spent in the quadrant where the original platform had been were markedly decreased compared with control group in spatial probe tests（P=0.047）.Protein expression of nAChRα4 in neurons of prefrontal cortex and hippocampus of rats decreased with hypoxia duration increasing.Conclusion Cognition disorders induced by intermittent hypoxia in rats probably correlated with decreased expression of nAChRα4 in neurons of prefrontal cortex and hippocampus.

关 键 词：缺氧 脑 认知障碍 烟碱型乙酰胆碱受体α4 大鼠 

分 类 号：R749.1[医药卫生—神经病学与精神病学]