尾斑瘰螈种群的D-loop区遗传多样性分析  

Genetic Diversity Analysis of D-loop Region in Populations of Paramesotriton caudopunclatus

作　　者：陈光照[1] 肖羽[1] 陈学平[1] 谷晓明[1] 

机构地区：[1]贵州师范大学生命科学学院,贵阳550001

出　　处：《动物学杂志》2011年第3期55-63,共9页Chinese Journal of Zoology

基　　金：贵州省教育厅自然科学研究项目(No.黔教科(2007)017);贵州省国际科技合作重点项目(No.黔科合外G字(2008)700116)

摘　　要：以线粒体DNA D-loop序列为分子标记,研究了采自雷公山和武陵山的尾斑瘰螈(Paramesotriton caudopunclatus)5个种群的遗传多样性。获得42个个体mtDNA D-loop区序列,长度为690 bp,其中有30个变异位点,由此定义了13个单倍型。5个种群中未出现共享单倍型的现象。尾斑瘰螈种群有相对较多的单倍型数(Hd)和较低的核苷酸多样性(Pi)。遗传分化指数(FST)、核苷酸歧异度(Dxy)和基因流(Nm)分析表明,雷公山的3个种群与武陵山的2个种群间有了一定程度分化。从系统进化树来看,各单倍型按照地理分布聚合,雷公山的3个种群构成一支,武陵山的2个种群构成另一支。建议将雷公山种群、武陵山种群分别作为2个不同单元进行保护。We studied the genetic diversity of five populations of Paramesotriton caudopunclatus using D-loop sequence of mtDNA as a molecular marker.As a result,we obtained 690 bp long D-loop sequences of 42 samples from five populations collected from Leigongshan and Wulingshan.In the sequences,there were 30 variable sites.Based on these sites 13 haplotypes are defined.No shared haplotype was found among the five populations.P.caudopunclatus populations had high haplotype diversity and low nucleotide diversity.The results of fixtion index（FST）,nucleotide diversity（Dxy） and migration rates（Nm） analyses indicated that a certain extent differentiation occurred between Leigongshan population and Wulingshan population.In phylogenetic trees,all haplotypes were clustered according to their geographic distribution.Three populations from Leigongshan constituted one clade,while the other two populations from Wulingshan constituted another clade,supporting the treatment of Leigongshan and Wulingshan populations as two separate management units.

关 键 词：尾斑瘰螈 地理种群 D-LOOP区 遗传多样性 

分 类 号：Q953[生物学—动物学]