高压氧对局灶性脑缺血再灌注大鼠脑组织小窝蛋白-1、基质金属蛋白酶-9表达的影响  

Effects of hyperbaric oxygen on caveolin-1 and matrix metalloproteinase-9 in brain tissues after cerebral focal ischemia-reperfusion in rats

作　　者：赵红[1] 张倩茹[1] 卢晓梅[2] 张海鹏[2] 陈学新[3] 

机构地区：[1]中国医科大学基础医学院机能实验中心,沈阳市和平区北二马路92号110001 [2]中国医科大学基础医学院病理生理教研室 [3]中国医科大学附属第一临床医院高压氧科

出　　处：《中国康复医学杂志》2011年第6期550-554,共5页Chinese Journal of Rehabilitation Medicine

基　　金：辽宁省教育厅高等学校科研基金资助项目(2005L456);辽宁省科技厅博士启动基金资助项目(20101149)

摘　　要：目的:研究高压氧(HBO)对局灶性脑缺血再灌注大鼠脑组织小窝蛋白-1(Cav-1)、MMP-9表达的影响。方法:雄性Wistar大鼠,随机分成假手术组、脑缺血再灌注组(IR)、IR+HBO、HBO组。复制局灶性脑缺血再灌注模型。HBO和IR+HBO组经0.25MPaHBO治疗5次。采用比色法、免疫组化法及Western印迹法检测血脑屏障的通透性和Cav-1、基质金属蛋白酶-9(MMP-9)蛋白的表达。结果:IR组第4、12、24、48、72小时脑组织伊文思蓝(EB)的含量与第0小时组相比明显增加,再灌注后第4小时EB的含量最高。IR+HBO组脑组织EB的含量明显低于IR组。IR组第4、12、24、48、72小时Cav-1、MMP-9蛋白表达明显高于0小时组。IR+HBO组与IR组相比脑组织Cav-1、MMP-9蛋白表达显著减低。结论:HBO降低了脑缺血再灌注时增高的脑微血管内皮细胞Cav-1蛋白和MMP-9的表达。这可能是HBO对脑缺血再灌注时血脑屏障通透性起保护作用的机制之一。Objective： To investigate the effect of hyperbaric oxygen （HBO） on caveolin-1（Cav-1） and matrix metalloproteinase-9（MMP-9） in brain tissues after cerebral focal ischemia-reperfusion in rats. Method： Male Wistar rat were randomly assigned into sham, ischemia-reperfusion （IR）, HBO, and IR＋HBO groups. On cerebral focal ischemia-reperfusion models, 0.25MPa HBO were applied 5 times, and 2% Evens blue （EB） was injected into tail veins 1h before execution. Expressions of Cav-1 and MMP-9, contents of EB in brain tissues were determined by Western blot technique, immunohistochemistry, and spectrophotometer, respectively. Result： In IR group, EB content in brain tissues increased significantly compared with 0h, reached it＇s peak at the 4th h（P0.01）. EB content in IR＋HBO group was significantly lower than that in IR group. While expressions of Cav-1 and MMP-9 augmented significantly by IR injury at the 4thh, 12thh, 24thh, 48thh, and 72ndh. Compared to IR group, HBO intervention reduced their corresponding expressions levels. Conclusion： HBO intervention can reverse the increase of Cav-1 and MMP-9 caused by IR injury, which maybe one of the mechanisms for the protective effect of HBO on the permeability of blood-brain barrier in IR injury.

关 键 词：高压氧 脑缺血再灌注 小窝蛋白-1 基质金属蛋白酶-9 

分 类 号：R743.3[医药卫生—神经病学与精神病学] R459.6[医药卫生—临床医学]