基底动脉中Caspase3、8的表达与蛛网膜下腔出血后脑血管痉挛的关系  

Relationship of expressions of Caspases 3 and 8 in basilar arteries with cerebral vasospasm after subarachnoid hemorrhage in rabbits

作　　者：张元隆[1,2] 王灯亮[1] 康德智[1] 游鸿海[1] 

机构地区：[1]福建医科大学第一临床医学院神经外科,福州350004 [2]福建医科大学

出　　处：《中国临床神经外科杂志》2011年第5期284-287,共4页Chinese Journal of Clinical Neurosurgery

基　　金：福建省科学技术厅重点项目(2009Y0018)

摘　　要：目的探讨天冬氨酸特异性半胱氨酸蛋白酶3、8(Caspase3、8)在蛛网膜下腔出血(SAH)后在基底动脉中的表达及其与脑血管痉挛的关系。方法新西兰大白兔36只,随机分成对照组(n=6)和实验组(n=30),后者再随机分为SAH后1、3、5、7、10d等5个亚组,每亚组各6只。采用枕大池二次注血法建立SAH模型,应用免疫组化和末端脱氧核苷酸转移酶介导的dUTP原位切口末端标记法分别检测基底动脉内皮细胞Caspase3、8表达和凋亡。结果凋亡细胞在实验组SAH后第1天出现,第7天凋亡水平达到最高。实验组Caspase3、8表达水平明显高于与对照组(P<0.05)。Caspase3、8的表达在SAH后第1天就可观察致到,第5天和第7天出现强烈表达,第l0天表达明显减弱。结论本结果提示在兔脑血管痉挛的基底动脉中存在细胞凋亡;Caspase3、8可能参与了SAH后脑血管痉挛的发生和发展。Objective To study the expressions of cysteine-dependent aspartate-specific proteases （caspases） 3 and 8 in the basilar artery after subarachnoid hemorrhage （SAH） and their relationship with cerebral vasospasm.Methods Thirty-six New Zealand rabbits were randomly divided into both the control group （n=6） and SAH groups （n=30）.Thirty rabbits in SAH group were randomly divided again into 5 subgroups of 1,3,5,7,10 days after SAH （6 per group）.Model of SAH was induced by injecting autologous arterial blood into the cisterna magna twice.The expressions of Caspase 3 and 8,and the apoptosis of endothelial cells in the basilar arteries were determined respectively by Immunohistochemistry technique and terminal-deoxynucleoitidyl transferase mediated nick end labeling technique.Results Apoptosis cells appeared in the SAH groups 1 day after SAH and reached the peak 7 days after SAH.The levels of Caspase 3 and 8 expression in the basilar arteries were significantly higher in SAH group 1,3,5,and 7 days after SAH than those in the control group （P 0.05）.The levels of Caspase 3 and 8 expressions in the basilar arteries reached the peak 5 and 7 days after SAH and were significantly decreased 10 days after SAH compared to those 7 days after SAH.Conclusions The apoptosis of the endothelial cells exists in the basilar arteries of rabbits with cerebral vasospasm,which may be related to the increase in the expressions of Caspases 3 and 8 in the basilar arteries.

关 键 词：蛛网膜下腔出血 CASPASE3 CASPASE8 凋亡 脑血管痉挛 

分 类 号：R743.35[医药卫生—神经病学与精神病学] Q786[医药卫生—临床医学]