自体CIK细胞输注治疗老年骨髓增生异常综合征的临床研究  

Clinical Study of Autologous Cytokine Induced Killer Cell Infusion Treating for Elderly Patients with Myelodysplastic Syndrome

作　　者：刘洋[1] 包尔宁[2] 杨波[1] 卢学春[1] 朱宏丽[1] 韩为东[3] 王瑶[3] 代汉仁[3] 姚善谦[1] 

机构地区：[1]解放军总医院南楼血液科住院病案管理科,北京100853 [2]解放军总医院住院病案管理科,北京100853 [3]解放军总医院基础医学研究所免疫室,北京100853

出　　处：《中国实验血液学杂志》2011年第3期787-792,共6页Journal of Experimental Hematology

基　　金：国家自然科学基金项目(编号30772597、30901187);中央保健研究专项基金(编号132009B115);军队“十一五”后勤科研计划课题(编号2006B);解放军总医院科技创新基金(编号ZY25、09KMM42)

摘　　要：本研究旨在评价自体细胞因子诱导的杀伤细胞(cytokine-induced killer cells,CIK)治疗老年骨髓增生异常综合征(myelodysplastic syndromes,MDS)的安全性和有效性。采集6例老年MDS患者外周血单个核细胞,在体外经细胞刺激因子培养,诱导成CIK细胞,回输至患者体内,28天为1个疗程。观察CIK细胞回输后患者体内效应细胞的比例变化、不良反应以及对感染的发生、血红蛋白水平和对输血依赖程度的影响。结果表明,经CIK细胞治疗后CD3+、CD3+CD8+、CD3+CD56+细胞比例明显升高(p<0.05),所有患者未出现严重不良反应。CIK细胞治疗有效地减少了MDS患者感染的发生,缩短了高热时间。在疾病稳定期,CIK细胞输注可减少红细胞的输注量,稳定血红蛋白水平,但不能改变MDS向高危亚型转化的自然病程。结论 :自体CIK细胞输注治疗老年人MDS安全有效。Objective of this study was to evaluate the effectiveness and safety of autologous cytokine induced killer （CIK） cells combined with IL-2 in treatment of elderly patients with myelodysplastic syndromes （MDS）. Peripheral blood mononuclear cells were isolated from 6 elder/y MDS patients and were stimulated by cytokines in vitro to form CIK cells. The autologous CIK cells were then infused back into the corresponding patients. The regimen was repeated every 4 weeks. Effector cell proportion changes, adverse effects, effects on inflammation, hemoglobin level and blood transfusion were assessed after treatment. The results showed that after autologous CIK cell infusion, the percentages of CD3 ＋ , CD3 ＋ CD8 ＋ and CD3 ＋ CD56 ＋ increased significantly （p 〈 0.05 ）. No severe adverse effects were observed in all patients. It also significantly reduced inflammation frequency and shortened high fever duration. During stable stage of disease, the CIK cell infusion could reduce the red blood cell infusion amount and stabilize hemoglobin level. However, the natural course of transformation from myelodysplastic syndromes to high-risk subtypes could not be changed by CIK cell treatment. It is concluded that the autologous CIK cell infusion is a safe and effective therapy for geriatric myelodysplastic syndrome.

关 键 词：细胞因子诱导的杀伤细胞 骨髓增生异常综合征 老年人 细胞输注 

分 类 号：R733.3[医药卫生—肿瘤] R733.7[医药卫生—临床医学]