三氧化二砷对食管癌细胞株Eca109的放射增敏作用及机制  

Radiosensitivity and Its Mechanism of Arsenic Trioxide on Esophageal Cancer Cell Line-Eca109

作　　者：景绍武[1] 王雅棣[1] 吴凤鹏[1] 卢付河[1] 韩春[1] 刘青[1] 程云杰[1] 

机构地区：[1]河北医科大学第四医院放疗科,石家庄050011

出　　处：《肿瘤防治研究》2011年第6期620-623,共4页Cancer Research on Prevention and Treatment

基　　金：国家自然科学基金资助项目(30540005);人事部高层次留学人才回国基金资助项目(国人厅发[2005]118号);教育部留学回国人员科研启动基金资助项目(教外司留[2008]101号)

摘　　要：目的探讨三氧化二砷(Arsenic trioxide,As2O3)及As2O3与放射线联合对食管癌Eca109细胞的增殖抑制作用;分析二者联合对Eca109细胞周期和凋亡的影响,为As2O3的临床应用提供理论依据。方法 MTT法观察放射线及As2O3单独及二者联合对食管癌Eca109细胞的增殖抑制作用,利用直线相关及直线回归拟合出照射组、As2O3组及联合组的直线,计算增敏比(sensitizing enhancement ra-tio,SER);流式细胞术检测As2O3联合放射线对Eca109细胞周期和凋亡的影响;Western blot印记技术观察As2O3联合放射线对锰超氧化物歧化酶(Manganese superoxide dismutase,MnSOD)和细胞色素C(cytochrome C,cyt-C)蛋白表达的影响。结果 As2O3能抑制食管癌Eca109细胞的增殖,具有明显的量效和时效关系;As2O3对食管癌Eca109细胞有明显的放射增敏作用;As2O3与放射线联合作用于Eca109细胞后,G2/M期细胞比例及凋亡率明显增加,MnSOD蛋白表达下降,而cyt-C蛋白表达未见明显变化。结论 As2O3可能通过G2/M期阻滞,降低MnSOD蛋白的表达,从而诱导细胞凋亡来增强放射线对细胞的杀伤作用。Objective To explore the effects of Arsenic trioxide（As2O3） and As2O3 combined with radiation on the growth inhibition of esophageal cancer cell line-Eca109.To analysis the impact of As2O3 combined with radiation on the cell cycle and apoptosis in order to provide a theoretical basis for As2O3 applied the treatment of esophageal cancer.Methods The inhibitory effects of radiation or/and As2O3 on proliferation were measured by MTT assay.The straight of irradiated group,As2O3 group and combined group were fitted by linear correlation and linear regression and then sensitizing enhancement ratio（SER） was calculated.The change of cell cycle distribution and apoptosis were assayed by flow cytometry.The effects of As2O3 combined with radiation on manganese superoxide dismutase（MnSOD） and cytochrome C（cyt-C） expression were measured by Western blot.Results With a clear dose and time effect,As2O3 could inhibit the proliferation of esophageal cell line-Eca109;As2O3 definitely enhanced radiosensitivity of Eca109 cells.Flow cytometry analysis results indicated that G2/M phase proportion and apoptosis rate increased obviously after the effect of As2O3 and radiation.The expression of MnSOD was down-regulated,while cyt-C had no obvious change.Conclusion As2O3 could enhance radiation＇s killing effect on Eca109 cells through G2/M phase arrest,down-regulation of MnSOD and induction of apoptosis.

关 键 词：三氧化二砷 凋亡 细胞周期 锰超氧化物歧化酶 

分 类 号：R735.1[医药卫生—肿瘤] R730.55[医药卫生—临床医学]