自体Retronectin诱导的CIK细胞治疗晚期结肠癌的疗效观察  

Therapeutic effects of autologous Retronectin activated CIK killer cell immunotherapy for patients with advanced colon carcinoma

作　　者：徐本玲[1,2] 高全立[1,3] 袁龙[1,4] 范瑞华[1,2] 张成娟[1,2] 宋永平[1,2] 

机构地区：[1]郑州大学附属肿瘤医院 [2]河南省肿瘤医院中心实验室,河南郑州450008 [3]河南省肿瘤医院生物治疗科,河南郑州450008 [4]河南省肿瘤医院普外科,河南郑州450008

出　　处：《基础医学与临床》2011年第7期783-787,共5页Basic and Clinical Medicine

基　　金：河南省医学科技攻关计划项目(201001012)

摘　　要：目的探讨Retronectin诱导的CIK治疗晚期结肠癌安全性及有效性。方法分离患者单个核细胞用Retrone-ctin、CD3单抗、IFN-γ和IL-2等细胞因子诱导产生CIK细胞。患者每周回输1次CIK细胞,连续4次。采用流式细胞术检测患者外周血淋巴细胞免疫表型,应用BDTM-HumanCBATh1/Th2型细胞因子试剂盒监测治疗前及回输后7天外周血细胞因子变化。结果每个患者平均回输细胞数为23.9×109/L(20.6×109～27.2×109),不良反应小。CIK细胞输注后,患者外周血中淋巴细胞数、CD3+、CD3+CD56+和CD3+CD8+细胞数均有明显升高(P<0.05)。12例患者中有1例获部分缓解(PR),4例稳定,其中PR和2例SD患者的CEA水平有明显下降(P<0.05)。治疗后临床获益患者的血清中IFN-γ(6.9vs53.2 pg/mL)和TNF-α(3.2vs24.6 pg/mL)明显升高(P<0.05)。结论自体Retronectin诱导的CIK细胞通过增加效应细胞数明显增强免疫功能而无不良反应,是一种安全有效的过继免疫治疗,对恶性肿瘤的传统治疗策略有一定的借鉴作用。Objective To study the therapeutic safety and effects of autologous Retronectin activated CIK cell immunotherapy for patients with advanced colon carcinoma.Methods CIK cells were isolated from peripheral blood mononuclear cells（PBMC） and incubated in the presence of IFN-γ followed by Retronectin,mouse antihuman CD3 monoclonal antibody and IL-2.Then,CIK cells were intravenously transfused every week for at least 4 weeks.Immunophenotypic characteristics of CIK cells were analyzed by flow cytometry.IFN-γ,tumour necrosis factor α（TNF-α）,IL-2,IL-10 and IL-4 concentrations in serum were determined with BDTM human Cytometric Bead Array kits（CBA）.Results The median number of transferred cells per patient was 23.9×109/L（20.6×109～27.2×109）,and the toxicity profile was favourable.After CIK cells infusion,the absolute median count of lymphocytes,CD3＋,CD8＋ and CD3＋CD56＋ cells significantly increased in patient＇s peripheral blood.One patients had partial response（PR） and four patients had stabilization of disease（SD）.The downtrend of the CEA level wasobserved in the PR and two SD patients.The patients with positive response after treatment increased the production of IFN-γ（6.9 vs 53.2 pg/mL） and TNF-α（3.2 vs 24.6 pg/mL）.No significant difference was seen in non-responders and in the expression of other cytokines.Conclusion Adoptive immunotherapy with Retronectin induces CIK cells and is a safe it could significantly enhance immune functions without side effects.

关 键 词：结肠癌 CIK 免疫功能 

分 类 号：R735.35[医药卫生—肿瘤]