人脂肪组织来源的神经干细胞移植治疗大鼠脑缺血再灌注损伤  

Transplantation of neural stem cells from human adipose tissue-derived stromal cells for the treatment of cerebral ischemic-reperfusion injury in rats

作　　者：刘斌[1] 董静[1] 刘宁[2] 王瑞敏[3] 张晋霞[1] 李世英[1] 陈贵良[4] 

机构地区：[1]河北联合大学附属医院神经内一科,河北唐山063000 [2]河北联合大学附属开滦医院急诊科,河北唐山063000 [3]河北联合大学实验中心,河北唐山063000 [4]河北联合大学实验动物中心,河北唐山063000

出　　处：《基础医学与临床》2011年第7期788-792,共5页Basic and Clinical Medicine

基　　金：河北省自然科学基金(C2008000994);河北省优秀专家出国培训基金(2007);唐山市科研基金(08130204a-1-21)

摘　　要：目的探讨人脂肪组织来源的神经干细胞移植治疗大鼠脑缺血再灌注损伤的疗效。方法 线栓法制作大鼠大脑中动脉缺血(MCAO)2 h再灌注模型。体外培养脂肪基质细胞,诱导分化为神经干细胞。移植治疗组经尾静脉移植人脂肪组织来源的神经干细胞悬液(2×106/mL)。用MNSS量表测定神经功能,TTC、HE染色观察脑梗死体积及脑组织病理变化。结果移植治疗组再灌注14、21和28 d的神经功能评分低于缺血再灌组(P<0.05或P<0.01)。移植治疗组第14天脑梗死体积(40.20±9.52 mm3)小于缺血对照组(66.60±14.24 mm3)(P<0.01)。移植治疗组的神经细胞变性、坏死数量较缺血再灌组明显减少。结论 人脂肪组织来源的神经干细胞移植治疗可改善脑缺血再灌注损伤大鼠的神经功能,具有神经保护作用。Objective To study the effects of neural stem cells from human adipose tissue-derived stromal cells transplantation for the treatment of cerebral ischemic-reperfusion injury in rats.Methods The middle cerebral artery occlusion（MCAO）2 h and reperfusion models were made by intraluminal vascular occlusion in rats.Adipose tissue-derived stromal cells were cultivated and induced into neural stem cells.Transplantation group was transplanted with neural stem cells（2×106 cells/mL） through tail vein at 24 h hours after MACO.Neurological function changes were recorded by using Modified neurologic severity scores（MNSS）,the volume of cerebral infarction and the change of cerebral pathology were observed by TTC and HE staining.Results The neurologic deficit scores in transplantation treated group were significantly lower than those in ischemia control group at 14 d,21 d,28 d of MCAO（P0.05,or P0.01）.The infarction volume of transplantation treated group（40.20±9.5 mm3） was less than that of ischemia control group（66.60±14.24 mm3） at 14 d（P0.01）.The number of degeneration and necrosis nerve cell in ischemia transplanted group is significantly reduced compered to ischemia-reperfusion group.Conclusion Transplantation of neural stem cells differentiated from human adipose tissue-derived stromal cells may improve neurologicalfunctionon of cerebral ischemic-reperfusion injury in rats and protective effect on neurons.

关 键 词：脂肪基质细胞 神经干细胞 脑缺血再灌注损伤 移植 

分 类 号：R741[医药卫生—神经病学与精神病学]