丙戊酸钠对Kasumi-1细胞裸鼠移植瘤细胞周期的影响  

Inhibitory effect of Valproic Acid on cell cycle of Kasumi-1 cell line in nude mice

作　　者：田霞[1] 刘鹏[1] 王丽红[1] 张志华[1] 赵蕾[1] 郝长来[1] 

机构地区：[1]承德医学院附属医院血液病科,河北承德067000

出　　处：《基础医学与临床》2011年第7期799-803,共5页Basic and Clinical Medicine

基　　金：河北省自然科学基金(C2008000660)

摘　　要：目的观察丙戊酸钠(VPA)对急性髓性白血病(AML)Kasumi-1细胞裸鼠移植瘤组织细胞周期的影响。方法建立Kasumi-1细胞裸鼠移植瘤模型,分为对照组和VPA治疗组,观察裸鼠移植瘤的生长情况,计算肿瘤体积和肿瘤生长抑制率。用流式细胞仪检测细胞周期,用免疫组化及Western blot检测P21WAF1/CIP和RB蛋白磷酸化(pRB)水平。结果 (1)VPA体内用药明显抑制裸鼠Kasumi-1细胞移植瘤的生长,瘤体积及重量明显小于对照组,肿瘤抑制率(IR%)为57.25%。(2)细胞周期阻滞于G0/G1期。(3)P21WAF1/CIP蛋白表达水平(0.900±0.113)显著高于对照组(0.390±0.001)、pRB蛋白表达水平(0.340±0.620)显著低于对照组(0.810±0.420)。结论 VPA可通过对Kasumi-1细胞周期蛋白及周期蛋白依赖性激酶抑制剂的影响,使细胞阻滞于G0/G1期。Objective To investigate the influence of Valproic acid（VPA） on cell cycle of Kasumi-1 cells.MethodsKasumi-1 cell line models transplanted subcutaneously in nude mice were established and divided into control group and VPA group to observe the growth of nude mice and their transplanted tumors Kasumi-1 cell of each group cell cycle changes were analyzed by flow cytometry.P21WAF1/CIPand pRB were detected by immunohistochemistry and Western blot.Results（1）VPA obviously inhibited the growth of tumor,animals treaed with VPA showed a statistically significant decrease in tumor volume and weight compared with controls with 57.25% growth inhibition.（2）VPA blocked cell in G0/G1 phase were detected by flow cytometry.（3）The P21WAF1/CIP protein significantly increased with VPA group.pRB protein significantly decrease with VPA group.Conclusion Administration of VPA inhibits the growth of Kasumi-1 cell line tumor VPA can arrest Kasumi-1 cell in Go/G1 phase through the regulation of P21WAF1/CIP and pRB.

关 键 词：白血病 丙戊酸钠 细胞周期 裸鼠移植瘤 磷酸化的视网膜母细胞瘤蛋白 

分 类 号：R733.71[医药卫生—肿瘤]