内吗啡肽对小鼠树突状细胞免疫功能的影响  

Effect of endomorphins on immunologic functions of mouse bone marrow-derived dendritic cells

作　　者：李正红[1] 于影[1] 杨锐[1] 方迎艳[1] 杨丽娟[1] 高琴[1] 

机构地区：[1]蚌埠医学院生理学教研室,安徽蚌埠233030

出　　处：《中国药理学通报》2011年第7期939-943,共5页Chinese Pharmacological Bulletin

基　　金：国家自然科学青年基金资助项目(No81000074);安徽高校省级自然科学重点资助项目(NoKJ2011A202)

摘　　要：目的研究内吗啡肽-1和内吗啡肽-2对小鼠骨髓来源树突状细胞表型和免疫功能的影响。方法从7～8周龄的C57BL/6J小鼠提取骨髓前体细胞培养,经CD11c免疫磁珠分选,获得纯化的树突状细胞(dendritic cells,DC)。未成熟DC用脂多糖或脂多糖+不同浓度内吗啡肽共培养,流式细胞术检测DC的表型;检测内吗啡肽对DC趋化性的影响。在DC和T淋巴细胞共培养的条件下,用氚标记胸腺嘧啶核苷(3H-TdR)参入测定的方法检测T淋巴细胞的增殖能力。结果内吗啡肽抑制DC的趋化性;与T淋巴细胞体外共培养时,经内吗啡肽-1或内吗啡肽-2处理的DC能抑制T淋巴细胞的增殖反应。上述内吗啡肽的作用都能被Mu阿片受体特异性拮抗剂CTOP翻转或部分翻转,提示内吗啡肽的作用是由Mu受体介导的。结论内吗啡肽可通过作用于Mu阿片受体,改变树突状细胞的部分免疫功能,调节免疫反应。Aim The aim of the present study was to investigate the effect of endomorphins on the immunologic functions of mouse bone marrow-derived dendritic cells.Methods Bone marrow dendritic cells were extracted from normal,7~8-week-old C57BL/6J mice,purified with anti-CD11c（a specific marker for dendritic cells） antibody-coated magnetic beads and cultured in the presence of GM-CSF.Then the immature DCs were cultured in the presence of LPS or LPS plus different concentration of endomorphins.DCs were analyzed for expression of the surface markers by flow cytometry.The effect of endomorphins on the DCs chemotaxis was investigated.3H-thymidine incorporation was used to detect the proliferation of T cells under the condition of co-culture of T lymphocytes with endomorphin-treated dendritic cells.Results Endomorphins inhibited DCs chemotaxis.Consistently endomorphins-treated dendritic cells inhibited the proliferation of T lymphocytes.All the above-mentioned effects of endomorphins could be reversed by CTOP（specific mu-opioid receptor antagonist）,suggesting that these effects of endomorphins were mediated by mu-opioid receptors.Conclusion Endomorphins modulate the functional properties of dendritic cells and immune response by acting on mu-opioid receptors.

关 键 词：内吗啡肽-1 内吗啡肽-2 Mu阿片受体 树突状细胞 免疫功能 抗原递呈细胞 趋化性 

分 类 号：R-332[医药卫生] R329.24