共刺激分子B7-H3和Ki-67在人脑胶质瘤中的表达及相关性  

Expressions and correlation of costimulatory molecule B7-H3 and Ki-67 in human glioma

作　　者：邢涛[1] 张利勇[1] 王丽[2] 朱建新[1] 

机构地区：[1]聊城市人民医院神经外科,252000 [2]潍坊市寒亭区人民医院检验科,261100

出　　处：《中华神经医学杂志》2011年第6期557-559,共3页Chinese Journal of Neuromedicine

摘　　要：目的研究共刺激分子B7-H3和Ki-67在脑胶质瘤中的表达及二者的相关性。方法选取聊城市人民医院神经外科自2008年3月至2010年1月间手术切除并经病理证实的人脑胶质瘤液氮冻存标本46例，其中低级别21例、高级别级25例，另取同期10例脑外伤或脑出血患者在手术非功能区入路过程中必须切除的正常脑组织作为对照。应用免疫组织化学染色检测各标本中B7-H3和Ki-67的表达。结果正常脑组织中B7-H3、Ki-67不表达，胶质瘤组织中B7-H3、Ki-67表达阳性率分别为65．22％、89．13％，与正常脑组织比较差异均有统计学意义（P〈0．05）。低、高级别脑胶质瘤之间B7-H3、Ki-67表达阳性率比较差异均无统计学意义（P〉O．05）。脑胶质瘤中B7-H3与Ki-67的表达呈正相关关系（F0．417，P=-0．014）。结论B7-H3和Ki-67是参与脑胶质瘤恶性发展的重要分子生物学因素，且两者可能存在协调作用。Objective To study the expressions of costimulatory molecule B7-H3 and Ki-67 in human glioma and explore their correlation. Methods Forty-six human brain gliomas, collected in our hospital from March 2008 to January 2010, including 21 with low grade and 25 with high grade, were chosen; and 10 normal tissues, collected from patients with cerebral trauma or cerebral hemorrhage at the same period, were chosen. Immunohistochemistry with SABC and SP methods were employed to detect the expressions of B7-H3 and Ki-67. Results B7-H3 and Ki-67 did not express in the normal brain tissues, but their expressions were detected in all glioma tissues of different grades （positive rate of BT-H3： 65.22%, that of Ki-67：89.13%）, which had significant differences （P〈0.05）. High-grade gliomas showed no significant differences on B7-H3 and Ki-67 expressions as compared with low-grade gliomas （P〉0.05）; and a positive correlation was noted between BT-H3 and Ki-67 expressions （r=0.417, P=0.014）. Conclusion Being the important molecular biological factors, B7-H3 and Ki-67 participate in the development of human glioma and exhibit a synergic effect in this process.

关 键 词：神经胶质瘤 B7-H3 KI-67 

分 类 号：R739.41[医药卫生—肿瘤]