栅藻LX1和雨生红球藻的胞外产物产生特性及其对藻细胞生长的影响  

The growth characteristic and the extracellular products of Scenedesmus sp.LX1 and Haematococcus pluvislis

作　　者：贾胜兰[1] 马小凡[1] 于茵[2] 李鑫[2] 胡洪营[2,3] 

机构地区：[1]吉林大学环境与资源学院,吉林长春130012 [2]清华大学环境学院//环境模拟与污染控制国家重点联合实验室,北京100084 [3]清华大学深圳研究生院,深圳518055

出　　处：《生态环境学报》2011年第4期635-639,共5页Ecology and Environmental Sciences

基　　金：国家杰出青年科学基金项目(50825801);国家"十一五"科技支撑计划项目(2007BAC22B02)

摘　　要：基于微藻在资源化大规模培养过程中,其胞外产物对藻细胞生长影响的不确定性,对比研究了两株高含油脂藻种,栅藻LX1(Scenedesmus sp.LX1)和雨生红球藻(Haematococcus pluvislis)的生长特征、胞外产物的产生特性,以及胞外产物对藻细胞生长的影响。研究结果表明,在相同培养条件下,栅藻LX1与雨生红球藻的内禀生长速率基本相当,分别为0.16和0.17 d-1。两株藻在不同生长期的胞外产物(以DOC值表征)产生速率不同,其中栅藻LX1胞外产物的对数期产生速率(1.4 mg·L-1·d-1)﹥稳定期后期产生速率(0.56 mg·L-1·d-1)﹥稳定期产生速率(0.48 mg·L-1·d-1);雨生红球藻胞外产物的稳定期后期产生速率(2.3 mg·L-1·d-1)﹥稳定期产生速率(0.88 mg·L-1·d-1)﹥对数期产生速率(0.66 mg·L-1·d-1)。栅藻LX1胞外产物对其整个生长过程都有明显抑制;雨生红球藻胞外产物则在稳定期后期才对其生长产生明显抑制作用。Scenedesmus sp.LX1 and Haematococcus pluvislis were chosen to study the growth characteristic and the production rate of extracellular products.We also cared about the influence of extracellular products on alga growth.The results indicated that： Haematococcus pluvialis had the similar specific growth rate（0.17 d-1） as Scenedesmus sp.LX1（0.16 d-1）.In different growth period,algae secreted extracellular products in different production rate.For Scenedesmus sp.LX1,the production rate at the later stationary phase（0.56 mg·L-1·d-1） was greater than that at stationary phase（0.48 mg·L-1·d-1）,and less than that at logarithmic phase（1.4 mg·L-1·d-1）.Referring to Haematococcus pluvislis,the production rate at stationary phase（0.88 mg·L-1·d-1） was greater than that at logarithmic phase（0.66 mg·L-1·d-1）,and less than that at later stationary phase（2.3 mg·L-1·d-1）.Extracellular products secreted by Scenedesmus sp.LX1 could inhibit cell growth through all the growth period.However,for the one of Haematococcus pluvislis,no inhibition was observed until latter stationary phase.

关 键 词：栅藻 雨生红球藻 生长特征 胞外产物 产生特性 

分 类 号：Q948.1[生物学—植物学] X173[环境科学与工程—环境科学]