不同剂量维生素D对哮喘大鼠肺组织eotaxin和CCR3的影响  

Effect of 1,25(OH)_2VitD_3 supplement on the production of cheomokine eotaxin and CCR3 in a rat asthma model

作　　者：刘佩意[1] 蒋卓勤[1] 

机构地区：[1]中山大学公共卫生学院营养学系,广东广州510080

出　　处：《热带医学杂志》2011年第5期529-531,535,F0004,共5页Journal of Tropical Medicine

摘　　要：目的观察早期补充不同剂量维生素D对哮喘大鼠肺组织CC族趋化因子嗜酸粒细胞激活趋化因子(eotaxin)和受体CCR3表达的影响。方法对用卵清蛋白(OVA)建立的大鼠哮喘模型进行1,25-(OH)2VitD3干预,分为低、中、高三个剂量,采用细胞计数、免疫组织化学染色和Westernblot方法检测哮喘大鼠肺组织炎症和eotaxin及CCR3的变化。结低中剂量维生素D可以减轻哮喘的炎性浸润,表现为肺泡灌洗液(BALF)中白细胞总数减少,嗜酸粒细胞(EOS)百分比下降,eotaxin和CCR3蛋白质表达减弱。相反,高剂量维生素D则促进哮喘的发作。结论早期补充低中剂量的维生素D对哮喘大鼠肺组织中eotaxin和CCR3有抑制作用,而高剂量维生素D则会促进eotaxin和CCR3的表达,从而加剧大鼠哮喘的气道炎症。Objective To evaluate the effect of vitamin D [1,25（OH）2D3] on the production of eotaxin and CCR3 in the lung issues and the airway inflammation in weaning rats with asthma.Methods Three different doses of vitamin D were administered intraperitoneally into rats sensitized with ovalbumin.After ovalbumin challenge,the bronchial alveolar lavage fluid（BALF） was collected and examined for the total cell number and eosinophil count.The lung tissue was removed and the degree of airway inflammation and expression of eotaxin and CCR3 was evaluated by immunohistochemistry and Western blot.Results Low to medium doses of vitamin D was found beneficial to reduce airway inflammation.The numbers of total BALF cell count and eosinophil were decreased and the expression of eotaxin and CCR3 was also decreased.However,a high dose of vitamin D had an opposite effect.Conclusion Administration of low to medium doses of vitamin D,at early stage after birth,may improve lung function and reduce the expression of eotaxin and CCR3 in the lung tissues of rats with asthma.However,high dose of vitamin D might cause frequent asthmatic attack and aggravate airway inflammation in asthmatic rats.

关 键 词：维生素D 哮喘 EOTAXIN CCR3 

分 类 号：Q565[生物学—生物化学] R562.25[医药卫生—呼吸系统]