3种方法检测口腔中幽门螺杆菌的效果  

Comparision of 3 Methods for Testing Helicobacter Pylori in Oral Cavity

作　　者：江毅[1] 陈天云[2] 危由春[2] 

机构地区：[1]中国人民解放军第一七一医院外四科,江西九江332000 [2]南昌大学第一附属医院口腔科,南昌330006

出　　处：《南昌大学学报（医学版）》2011年第2期60-62,共3页Journal of Nanchang University：Medical Sciences

摘　　要：目的比较快速尿素酶、多聚酶链反应(PCR)及细菌培养法检测胃炎或消化性溃疡患者口腔中的幽门螺杆菌(Hp)的效果。方法选取经胃镜证实为慢性胃炎或消化性溃疡的患者50例,在磨牙区刮取3份龈下菌斑,分别采用快速尿素酶、PCR、细菌培养法测定Hp。结果快速尿素酶、PCR、细菌培养法的Hp阳性率分别为80%、64%、26%,3种方法比较差异有统计学意义(P<0.05)。结论快速尿素酶、PCR、细菌培养法检测口腔中Hp有明显差异性,其中PCR法不仅特异敏感,而且速度较快、准确率高,是县及县以上医院较好的检测口腔中Hp的方法。Objective To compared the effects of rapid urease test,PCR and germiculture on detection of helicobacter pylori（Hp） in oral cavity and peptic ulcer.Methods Fifty patients with chronic gastritis or peptic ulcer confirmed by gastroscopy were randomly selected.Dental plaque samples were taken from molars and Hp was detected by rapid urease test,PCR and germiculture.Results Hp positive rates in dental plaque were 80%,64% and 26% by rapid urease test,PCR and germiculture,respectively.There were significant differences among the three methods（P0.05）.Conclusion PCR method is specific,sensitive,fast and accurate,and is applicable to hospitals at county level or above for testing Hp in oral cavity.

关 键 词：幽门螺杆菌 牙菌斑 快速尿素酶 多聚酶链反应 细菌培养 

分 类 号：R780.2[医药卫生—口腔医学]