胆囊良恶性病变组织中gal-3和SNA凝集素表达水平研究  

Expressions of galectin-3（gal-3） and sambucus nigra agglutinin （SNA） and their clinicopathological significances in benign and malignant lesions of the gallbladder

作　　者：杨乐平[1] 杨竹林[1] 刘洁琼[1] 杨晓静[2] 苗雄鹰[1] 

机构地区：[1]中南大学湘雅二医院肝胆疾病研究室,长沙410011 [2]中南大学湘雅二医院病理学系,长沙410011

出　　处：《中华肝胆外科杂志》2011年第6期484-487,共4页Chinese Journal of Hepatobiliary Surgery

摘　　要：目的研究胆囊良恶性病变组织中β-半乳糖凝集素-3（galectin-3，gal-3）和接骨木凝集素（sambucus nigra agglutinin，SNA）表达水平及其临床病理意义。方法108例胆囊腺癌、46例癌旁组织、15例腺瘤性息肉和35例慢性胆囊炎组织常规制作石蜡包埋切片。gal-3染色方法为EnVsion^TM免疫组织化学法，SNA染色法为ABC亲和组化法。结果胆囊腺癌gal-3和SNA表达阳性率（62．0％，66．7％）明显高于癌旁组织（39．1％，45．6％）、腺瘤性息肉（26．7％，33．3％）和慢性胆囊炎胆囊上皮（11．4％，11．4％），组间差异具有统计学意义（P〈0．05或P〈0．01）；gal-3和SNA表达阳性的良性病变胆囊上皮均呈不典型增生；高分化腺癌、肿块最大径〈2cm、无淋巴结转移及未侵犯周围组织病例gal-3和SNA表达阳性率均明显低于低分化腺癌、肿块最大径≥2cm、淋巴结转移及侵犯周围组织病例，组间差异具有统计学意义（P〈O．05或P〈0．01）；胆囊腺癌中gal-3和SNA表达水平呈高度一致性（X^2-9．51，P％0．01）。结论gal-3和SNA表达水平在胆囊腺癌发生、进展、临床生物学行为方面可能有重要作用，gal-3和（或）SNA阳性表达的胆囊腺癌可能预后不良。Objective To study the expressive levels of galectin-3 （gal-3） and sambucus nigra agglutinin（SNA） and to detect their clinicopathological significances in benign and malignant lesions of the gallbladder. Methods EnVisonTM immunohistochemistry for assaying gal-3 expressive levels and ABC cytochemistry for determining SNA expressive levels were used in conventional paraffin-embedded sections from the specimens of adenoearcinoma （n= 108）, peritumoral tissues （n=46）, adenomatous polyp （n= 15）, and chronic eholecystitis （n= 35）. Results The positive rates of gal-3 and SNA were significantly higher in adenoearcinoma （62.0 %, 66.7 % ） than in peritumoral tissues （39.1%, 45.6%）, adenomatous polyp （ 26.7%, 33.3%） and chronic cholecystitis （ 11.4%, 11.4%） （P〈0.05 or P〈0.01）. The positive gal-3 and SNA in benign cases showed atypical hyperplasia of the epthelium. The positive rates of gal-3 and SNA expression were significantly lower in well-differentiated adenoearcioma, mass with a maximal diameter of 〈2 cm, absence of lymph node metastasis, and absence of invasion to adjacent tissues than in poor-differentiated adenoearcinoma, mass with a maximal diameter of ≥2 cm, lymph node metastasis and invasion to adjacent tissues. （P〈0. 05 or P〈0.01）. There was a significant correlation between the expressive levels of gal-3 and SNA in gallbladder adenoeareinoma （X^2 = 9.51, P〈0.01）. Conclusions The expressive levels of gal-3 and SNA lectins had important effects on carcinogenesis, progression and biologic behaviors of gallbladder cancer. Patients with positive gal-3 and/or SNA expressions had poor prognosis.

关 键 词：胆囊肿瘤 息肉 慢性胆囊炎 β-半乳糖凝集素 接骨木凝集素 

分 类 号：R735.8[医药卫生—肿瘤]