经典型霍奇金淋巴瘤肿瘤细胞和背景淋巴细胞相关标记的表达  

Expressions of relative markers of neoplastic cells and background lymphoid cells in classical Hodgkin lymphoma

作　　者：王英[1] 杨华[1] 王连友[1] 崔亚南[1] 张丽荣[1] 

机构地区：[1]吉林大学中日联谊医院病理科,吉林长春130033

出　　处：《吉林大学学报（医学版）》2011年第3期509-512,F0003,共5页Journal of Jilin University：Medicine Edition

基　　金：卫生部科教司科研基金资助课题(1994-2-158);吉林省长春市科技局科研基金资助课题(2001-086-510)

摘　　要：目的:探讨经典型霍奇金淋巴瘤中的肿瘤细胞和非肿瘤背景淋巴细胞蛋白表达的特征及差异性。方法:收集经典型霍奇金淋巴瘤68例,对诊断切片和免疫标记片重新复查,采用Envision法补做相关免疫组织化学标记,观察CD30、CD15及T/B表型等的表达。结果:肿瘤细胞和背景淋巴细胞CD30的表达率分别为86.76%(59/68)和20.59%(14/68),CD15的表达率分别为51.47%(35/68)和63.24%(43/68);肿瘤细胞表达T细胞表型的阳性率为45.59%(31/68),B细胞表型的阳性率为42.65%(29/68)。成熟淋巴细胞以T细胞为主时,肿瘤细胞表达B细胞表型21例,表达T细胞表型28例;成熟淋巴细胞以非T细胞为主时,肿瘤细胞表达B细胞表型8例,表达T细胞表型3例(P>0.05)。结论:CD30和CD15是非特异性标记物,在肿瘤细胞和反应性淋巴细胞中均可表达;肿瘤细胞表达T细胞的免疫表型高于表达B细胞的免疫表型;背景细胞以T淋巴细胞为主时,肿瘤细胞表达T和B表型阳性率无差异。Objective To investigate the feature and difference of protein expression in neoplastic cells and non-neoplastic background lymphoid cells of classical Hodgkin lymphoma.Methods The clinical data,histological features and immunohistochemical findings of sixty-eight cases of classical Hodgkin lymphoma were collected.The additional immunostaining was performed if conformed by Envision method,the CD30,CD15 and T/B phenotype and so forth were mainly observed.Results The expression rates of CD30 in neoplastic cells and background lymphoid cells were 86.76%（59/68）and 20.59%（14/68）.The expression rates of CD15 in neoplastic cells and background lymphoid cells were 51.47%（35/68）and 63.24%（43/68）.The positive rates of T-cell and B-cell phenotypes in neoplastic cells were 45.59%（31/68） and 42.65%（29/68）.The expressions of B-cell and T-cell phenotype in neoplastic cells were 21 cases and 28 cases while mature lymphoid cells mainly expressed T-cell phenotype.The expressions of B-cell and T-cell phenotypes in neoplastic cells were 8 cases and 3 cases while mature lymphoid cells mainly expressed non T-cell phenotype.Conclusion Both CD30 and CD15 are not specific markers,they could express both in neoplastic cells and reactive lymphocytes.The T-cell phenotype expressed in neoplastic cells is higher than B-cell phenotype.There is no difference of positive rate between T-cell or B-cell phenotype in neoplastic cells while background cells mainly express T-cell phenotype.

关 键 词：经典型霍奇金淋巴瘤/病理学 肿瘤细胞 淋巴细胞 免疫表型 

分 类 号：R733[医药卫生—肿瘤]