雌激素对结肠细胞错配修复活性的影响  

Effect of estrogen on DNA mismatch repair activity in colonic cells

作　　者：牟韶娇[1,2] 盛剑秋[1] 谢惠[1] 金鹏[1] 陆晓娟[1] 高巍[1] 

机构地区：[1]北京军区总医院消化内科,北京100700 [2]山西医科大学

出　　处：《胃肠病学和肝病学杂志》2011年第5期440-442,共3页Chinese Journal of Gastroenterology and Hepatology

基　　金：国家自然科学基金(81072041)资助

摘　　要：目的观察雌激素对结肠细胞错配修复(MMR)活性的影响。方法在质粒pEGFP-N1的增强型绿色荧光蛋白(EGFP)基因起始密码子上引入错配,用含错配EGFP的质粒转染细胞,用不同浓度的雌激素干预转染错配EGFP质粒后的结肠癌细胞株SW 480,然后在流式细胞仪下观察绿色荧光细胞的数量和荧光强度,以反映雌激素对结肠细胞MMR活性的影响。结果雌激素能增强转染错配EGFP质粒后SW 480细胞中绿色荧光细胞的数量和荧光强度。结论雌激素能上调结肠细胞MMR活性。Objective To investigate the effect of estrogen on mismatch repair（MMR） activity in colonic cells.Methods AT-G mismatch was introduced into the ATG start codon of the enhanced green fluorescent protein（EGFP） gene in pEGFP-N1 plasmid.The MMR activity can be determined by measuring the intensity of the green fluorescence.Colonic cancer cell line SW480 was confected with this mismatched plasmid and different concentrational estrodiol was added into the medium.Then the intensity of the green fluorescence was detected by flow cytometry.Results Estrogen could increase the number of green fluorescent cells and enhance the intensity of the fluorescence.Conclusion Estrogen can up-regulate the MMR activity.

关 键 词：雌激素 错配修复 结肠细胞 

分 类 号：R574.62[医药卫生—消化系统]