抗菌肽SMAP-29结构功能及分子设计策略  

Structure,function and molecular design strategies of antibacterial peptide SMAP-29:a review

作　　者：陈琛[1] 吴三桥[1] 李新生[1] 张小莺[2] 闫茂仓[3] 

机构地区：[1]陕西理工学院陕西省资源生物重点实验室,汉中723000 [2]西北农林科技大学动物医学院,杨凌712100 [3]浙江省海洋水产养殖研究所,温州325005

出　　处：《生物工程学报》2011年第6期846-859,共14页Chinese Journal of Biotechnology

基　　金：陕西省教育厅科学研究计划(No.09JK373);温州市科技计划项目(No.S20100016)资助~~

摘　　要：抗菌肽是生物体内产生的一种具有生物活性的小分子多肽,具有广谱抗细菌、抗病毒、抗真菌甚至抗癌作用。SMAP-29是来源于绵羊骨髓细胞,包含29个氨基酸的Cathelicidin类α-螺旋结构抗菌肽。SMAP-29具有多种生物活性,包括抗革兰氏阳/阴性菌、抗真菌、抗病毒、抗寄生虫、抗螺旋体、抗衣原体和中和内毒素活性,并且具有作用机制独特、快速杀灭细菌的特点。以下综述了SMAP-29抗菌肽家族的基因和蛋白结构、结构与活性关系、作用机制、生物功能、基因重组表达,重点阐述了SMAP-29结构、分子设计的必要性和基于SMAP-29一级、二级结构进行分子设计策略,为SMAP-29药物设计和研究开发奠定了基础。Antibacterial peptides are a family of host-defense peptides most of which are gene-encoded and produced by living organisms of all types.Antibacterial peptides are small molecular proteins with broad antimicrobial spectrum against bacteria,viruses,fungi and sometimes even as anticancer peptide.SMAP-29,a cathelicidin-like peptide derived from sheep myeloid,line α-helical Structure,exerts a powerful broad antimicrobial activity against different pathogens including Gram-positive and Gram-negative bacteria,fungi,viruses,parasites,spirochaetes,chlamydia and antiendotoxin activity,and particular antibacterial mechanism,rapidly to permeabilize membranes of susceptible organisms.This paper summarizes the lately research progress of SMAP-29 and Variants including the characteristics of structure,structure-activity relationships,mode of action,diverse biological functions,gene recombinant and expression.We put emphasis on the necessity of molecular design,and primary and secondary structure-based modification,to provides a strong foundation for further drug development and design of SMAP-29.

关 键 词：抗菌肽 SMAP-29 活性 结构与功能 分子设计 

分 类 号：Q51[生物学—生物化学]