银杏外种皮多糖对人宫颈癌细胞系Siha增殖及侵袭的影响  

Effects of Ginkgo biloba exocarp polysaccharides on proliferation and invasion of cervix cancer cells line siha

作　　者：杨滨[1] 娄晓明[1] 吴春丽 李婉萍[1] 

机构地区：[1]中国医科大学附属盛京医院妇产科,沈阳110004 [2]盘锦市辽河油田二院妇产科,盘锦124010

出　　处：《实用药物与临床》2011年第3期179-181,共3页Practical Pharmacy and Clinical Remedies

基　　金：辽宁省教育厅高等学校科学技术研究项目(编号:2008802)

摘　　要：目的研究银杏外种皮多糖(Ginkgo biloba exocarppolysaccharides,GBEP)对宫颈癌细胞生长及增殖的影响。方法通过经典方法提取银杏外种皮多糖,通过MTT方法检测GBEP对处理宫颈癌细胞增殖的影响;Transwell检测GBFP对宫颈癌细胞迁移的作用;通过Real time PCR和ELISA检测细胞迁移相关蛋白MMP-2的表达。结果银杏外种皮多糖处理后的宫颈癌细胞增殖抑制率上升,与对照组比较,差异有统计学意义。银杏外种皮多糖用药后,迁移能力降低,MMP-2基因表达下降。结论银杏外种皮多糖对宫颈癌细胞的增殖和迁移可能会起到抑制作用。Objective To investigate the effect of proliferation and invasion on Siha with Ginkgo biloba exocarp polysaccharides.Methods The proliferation and invasion of Ginkgo biloba exocarp polysaccharides on Siha were reduced by MTT and Transwell.The expression of MMP-2 was examined by real time PCR and ELISA.Results For Siha cell with the increasing of Ginkgo biloba exocarp polysaccharides concentrations,the inhibitory rate increased.Moreover,Ginkgo biloba exocarp polysaccharides notably decreased the secretion of MMP-2.Conclusion The results indicated that Ginkgo biloba exocarp polysaccharides might be valuable in cervix cancer.

关 键 词：银杏外种皮多糖 细胞增殖 宫颈癌 

分 类 号：R737.33[医药卫生—肿瘤]