TRAIL联合吉西他滨对肺癌NCI-H460细胞增殖和凋亡的影响  

Effect of gemcitabine combined with TRAIL on proliferation and apoptosis of lung cancer NCI-H460 cells

作　　者：孙非凡[1] 孙铭娟[2] 王梁华[2] 焦炳华[2] 

机构地区：[1]第二军医大学研究生管理大队,上海200433 [2]第二军医大学基础部生物化学与分子生物学教研室,上海200433

出　　处：《中国肿瘤生物治疗杂志》2011年第3期296-300,共5页Chinese Journal of Cancer Biotherapy

基　　金：科技部"重大新药创制"科技重大专项(No.2009ZX09103-689);第二军医大学大学生创新能力培养研究课题资助项目(No.MS2009033)~~

摘　　要：目的：观察吉西他滨（gemcitabine）联合TNF相关凋亡诱导配体（TNF-related apoptosis inducing ligand,TRAIL）对人肺癌细胞NCI-H460增殖和凋亡的影响。方法：MTT法检测NCI-H460细胞的增殖,流式细胞术检测NCI-H460细胞的凋亡。结果：吉西他滨和TRAIL单用或联用均可浓度（0.00110μg/ml）和时间（24、48、72 h）依赖性抑制NCI-H460细胞的增殖,两药联用比单用时抑制率更高,3组药物作用72 h时对肿瘤的抑制率分别为14.9%77.5%、23.4%74.8%、22.3%80.5%;作用72 h时两药单用和联用对细胞增殖抑制的IC50分别为0.085 3、0.098 2、0.043 5μg/ml。两药联用对NCI-H460细胞增殖的抑制效果还与用药顺序以及两药比例有关,吉西他滨作用24 h后再加TRAIL比TRAIL作用24 h后再加吉西他滨对NCI-H460的抑制效果更好,吉西他滨与TRAIL联用的比例为1∶0.3时对NCI-H460细胞增殖的抑制率最大。吉西他滨和TRAIL联用时NCI-H460细胞的凋亡率显著高于两药单用的凋亡率（49.04%vs29.33%、25.69%,P〈0.01）。结论：吉西他滨与TRAIL联用可协同抑制肺癌细胞NCI-H460的增殖、促进细胞凋亡。Objective：To study the effect of gemcitabine combined with TRAIL（TNF-related apoptosis inducing ligand） on proliferation and apoptosis of human lung cancer NCI-H460 cells.Methods： MTT assay was used to analyze the proliferation of NCI-H460 cells,and flow cytometry was used to examine the apoptosis of NCI-H460 cells.Results： Gemcitabine or TRAIL alone or in combination inhibited the proliferation of NCI-H460 cells in a time-（24,48,72 h） and concentration-dependent（0.001-10 μg/ml） manner.Gemcitabine combined with TRAIL had a stronger inhibitory effect than they were used alone,with the inhibitory ratios being 14.9%-77.5%,23.4%-74.8%,and 22.3%-80.5% after 72 h in combination,gemcitabine,and TRAIL groups,and the IC50 being 0.0853,0.0982,and 0.0435 μg/ml respectively.The inhibitory effect of gemcitabine combined with TRAIL on proliferation of NCI-H460 cells was related to the administration order and the ratio of gemcitabine and TRAIL;gemcitabine treatment for 24 h and then giving TRAIL had a stronger inhibitory effect than TRAIL treatment for 24 h and then giving gemcitabine;when the ratio of gemcitabine and TRAIL was 1：0.3,the combination showed the strongest inhibitory effect.The apoptosis rate of NCI-H460 cells in the combination treatment group was significantly higher than in the other two groups（49.04% vs 29.33%,25.69%,P〈0.01）.Conclusion： Gemcitabine and TRAIL have synergistic effects in inhibiting proliferation and promoting apoptosis of lung cancer NCI-H460 cells.

关 键 词：吉西他滨 TNF相关凋亡诱导配体 人肺癌细胞 NCI-H460 增殖 凋亡 

分 类 号：R734.2[医药卫生—肿瘤] R730.5[医药卫生—临床医学]