胃癌患者外周血和肿瘤组织中高表达CD14^+DR^(low/-)髓源抑制性细胞  

Myeloid-derived suppressor cells highly expressing CD14^+DR^(low/-)in peripheral blood and tumor tissues of stomach carcinoma

作　　者：戴夫 李海文 彭琼 林沪[2] 李元元[2] 

机构地区：[1]安徽医科大学第三附属医院消化内科,安徽合肥230061 [2]解放军第302医院生物治疗中心,北京100039

出　　处：《中国肿瘤生物治疗杂志》2011年第3期311-314,共4页Chinese Journal of Cancer Biotherapy

基　　金：国家"十一五"科技重大专项(No.2009ZX10004-309)~~

摘　　要：目的:探讨胃癌患者外周血和肿瘤组织中CD14+DRlow/-髓源抑制性细胞(myeloid-derived suppressor cells,MDSCs)的表达及其与肿瘤病理特征的关系。方法:选取2009年3月至2010年10月安徽医科大学第三附属医院胃癌患者43例(Ⅰ期9例、Ⅱ期13例、Ⅲ期14例、Ⅳ期7例),另采集26例正常健康者作为对照组。流式细胞术检测外周血、瘤组织中CD14+DRlow/-MDSCs的表达水平,分析MDSCs表达水平与肿瘤病理特征的相关性。结果:胃癌患者肿瘤组织中CD14+DRlow/-MD-SCs的表达显著高于自身外周和健康对照者外周血的表达水平[2.87±1.93)%vs(2.37±1.7)%,(0.89±0.47)%,P<0.05和P<0.01],后两者间差异也有统计学意义(P<0.01)。CD14+DRlow/-MDSCs的表达与胃癌的恶性程度呈正相关,晚期胃癌组织内CD14+DRlow/-MDSCs表达明显增加(Ⅰ∶Ⅱ∶Ⅲ∶Ⅳ=(1.15±0.78)%∶(1.71±0.92)%∶(2.25±1.24)%∶(4.85±2.37)%,P<0.05)。同时,肿瘤浸润组织与非浸润组织的CD14+DRlow/-MDSCs表达也有明显差异[3.90±1.67)%vs(2.62±1.53)%,P<0.05]。结论:CD14+DRlow/-MDSCs在胃癌患者外周血和肿瘤组织中均高表达,与胃癌的发生、发展密切相关。Objective：To investigate the expression of CD14＋DRlow/-myeloid-derived suppressor cells（MDSCs） in peripheral blood and tumor tissues of gastric carcinoma（GC） patients and its relationship with clinicopathological of GC.Methods： Forty-three stomach carcinoma patients（9 stageⅠ,13 stageⅡ,14 stageⅢ,7stageⅣ） were selected from Third Affiliated Hospital of Anhui Medical University（Mar.2009 to Oct.2010）,and 26 healthy volunteers were used as control.CD14＋DRlow/-MDSCs expression in peripheral blood and gastric carcinoma tissues was detected by flow cytometry,and its relationship with clinicopathological of GC was analyzed.Results： CD14＋DRlow/-MDSCs expression in GC tissues was significantly higher than those in peripheral blood of GC patients and healthy controls（[2.87±1.93]% vs [2.37±1.7]%,[0.89±0.47]%,P〈0.05 and P〈0.01）,and CD14＋DRlow/-MDSCs expression in peripheral blood of GC patients was also higher than that in healthy controls（P〈0.01）.CD14＋DRlow/-MDSCs expression in GC tissues was positively correlated with their malignancy stage and significantly increased in advanced GC（stage Ⅰ∶Ⅱ∶Ⅲ∶Ⅳ=（1.15±0.78）%∶（1.71±0.92）%∶（2.25±1.24）%∶（4.85±2.37）%,P〈0.05）.Meanwhile,CD14＋DRlow/-MDSCs expression in tumor infiltration tissues was significantly higher than that in tumor un-infiltration tissues（[3.90±1.67）% vs [2.62±1.53]%,P〈0.05）.Conclusion： CD14＋DRlow/-MDSCs are highly expressed in peripheral blood and GC tissues,and relates to the development and progression of GC.

关 键 词：胃癌 髓源的抑制性细胞 免疫逃逸 免疫监视 

分 类 号：R735.2[医药卫生—肿瘤] R730.2[医药卫生—临床医学]