经颅电刺激对脑缺血再灌注大鼠运动功能和微管相关蛋白－2、生长相关蛋白－43的影响  

The effect of transcranial electrical stimulation on functional recovery and the expression of microtubule-associated protein-2 and growth -associated protein-43 after cerebral focal ischemia

作　　者：杨丽霞[1] 刘芳[2] 陈正红 

机构地区：[1]贵阳医学院附属医院心理科,贵阳550004 [2]贵阳医学院附属医院神经科,贵阳550004 [3]贵州省交通医院内科

出　　处：《中华物理医学与康复杂志》2011年第6期404-407,共4页Chinese Journal of Physical Medicine and Rehabilitation

摘　　要：目的研究经颅电刺激对局部脑缺血再灌注大鼠运动功能恢复的影响并从神经可塑性角度探讨其机制。方法将72只雄性Sprague－Daw｜ey大鼠按完全随机分组法分为电刺激组、模型组、假手术组和正常组，采用线栓法制备短暂性大脑中动脉缺血再灌注模型，造模后24h电刺激组给予经颅电刺激。分别于造模后第3、7、14和28天采用前肢放置试验（FPT）和走横木试验（BWT）进行评分，采用免疫组织化学法检测微管相关蛋白－2（MAP－2）和生长相关蛋白-43（GAP-d3）在梗死灶周围的灰度值。结果电刺激组FPT和BWT评分在第7、14、28天优于模型组，差异具有统计学意义（P〈0．05）；电刺激组梗死灶周围MAP-2的表达在第14天、28天高于模型组（P〈0．01）；电刺激组梗死灶周围GAP-43的表达在第3天、7天和14天高于模型组（P〈0．05）。结论经颅电刺激能促进脑缺血大鼠肢体功能恢复，上调梗死灶周围MAP-2和GAP43的表达。Objective To assess the influence of transeranial electric stimulation （TES） on the recovery of motor function after cerebral focal ischemia and reperfusion and to explore the mechanisms in terms of neural plasticity. Methods An acute focal ischemia-reperfusion model was established by transient occlusion of the right middle cerebral artery （MCAO）. Seventy-two male Sprague-Dawley rats were randomly divided into a TES group, a model group, a sham-operation group and a normal group. The TES group was given TES 24 h after MCAO; the model group received the operation without any treatment. Forelimb placing （FPT） and beam walking （BWT） were measured at the 3rd, 7th, 14th and 28th day after reperfusion. Microtubule-associated protein-2 （MAP-2） and growthassociated protein-43 （ GAP-43 ） and grey levels of reaction products in the peri-infarct region were examined by immunohistochemical techniques. Results The TES group rats had markedly better FPT and BWT performance at the 7th, 14th and 28th day after MCAO, compared with the model group. Expression of MAP-2 had increased significantly more at the 14th and 28th day in the peri-infaret region in the TES group compared with the model group. Expression of GAP-43 was significantly elevated in the peri-infarct region in the TES group compared with the model group at all time points. Conclusions TES can improve motor function and neural plasticity following cerebral ischemia and reperfusion damage. The functional enhancement may be partly due to up-regulation of the expression of GAP-43 and MAP-2 in the Deri-infarct region.

关 键 词：脑缺血再灌注 经颅电刺激 微管相关蛋白-2 生长相关蛋白-43 前肢放置试验 走横木试验 

分 类 号：R743.3[医药卫生—神经病学与精神病学]