WEE1与肝癌的关系  

Relationship between WEE 1 and hepatocellular carcinoma

作　　者：吕慧[1,2] 杨玉秀[2] 张立达[2] 白阳秋[2] 

机构地区：[1]郑州大学第一附属医院,河南省郑州市450003 [2]河南省人民医院消化内科,河南省郑州市450003

出　　处：《世界华人消化杂志》2011年第14期1515-1519,共5页World Chinese Journal of Digestology

基　　金：河南省医学科技攻关重点基金资助项目;No.200902002~~

摘　　要：目的:探讨WEE1在肝细胞癌(HCC)发生、发展中的异常表达及与肿瘤病理分期的关系.方法:收集2010-03/2010-05河南省人民医院肝胆外科手术标本正常肝组织23例,肝硬化20例,HCC42例.采用RT-PCR检测mRNA水平的表达,Western blot及免疫组织化学检测蛋白的表达,并分析其与肝癌临床病理学分期的关系.结果:WEE1mRNA阳性表达率分别为21.7%、55.0%和90.5%,三组间差异有统计学意义(P<0.01).Western blot和免疫组织化学方法分别检测蛋白阳性表达率分别为13.04%/17.4%、40%/60%和78.6%/83.3%,三组间相比差异有统计学意义(P<0.01).肝癌组织中WEE1强度与肿瘤的分化程度及病理分级相关(χ2=17.454,P<0.01;χ2=14.559,P<0.01).结论:WEE1基因在肝癌中呈上调表达,其参与的DNA的修复异常与肝癌的发生密切相关;;且与肿瘤的分化程度和病理分级相关.AIM：To investigate the expression of WEE 1 in hepatocellular carcinoma（HCC）and to analyze its relationship with clinicopathological characteristics of HCC.METHODS：Twenty-three normal human liver tissue specimens,20 cirrhosis specimens,and 42 HCC specimens were used in this study.Reverse transcriptional-polymerase chain reaction（RT-PCR）was used to measure the expression of WEE 1 mRNA in the above tissue specimens,while Western blot and immunohistochemistry were used to detect the expression of WEE 1 protein.The relationship between WEE 1 expression and clinicopathological characteristics of HCC was analyzed.RESULTS：The positive rates of Wee 1 mRNA expression in normal liver tissue,cirrhosis andHCC were 21.7%,55%and 90.5%,respectively,with a significant difference among the three groups（P0.01）.The positive rates of Wee 1 protein expression as revealed by Western blot and immunohistochemistry in the above three groups were 13.04%/17.4%,40%/60%and 78.6%/83.3%,respectively,with significant differences among the three groups（both P0.01）.Up-regulated expression of WEE 1 was significantly correlated with tumor differentiation and pathological grade in HCC（χ2=17.454,P0.01;χ2=14.559,P0.01）.CONCLUSION：WEE 1 expression was significantly upregulated in HCC.High expression of WEE 1 may be closely related with tumor differentiation and pathological grade in HCC.

关 键 词：逆转录聚合酶链式反应 免疫蛋白印迹 免疫组织化学 肝细胞癌 WEE1 

分 类 号：R735.7[医药卫生—肿瘤]