hARD1在人大肠腺癌中的表达及与肿瘤分化的相关性  

Correlation between hARD1 expression and tumor differentiation in human colorectal carcinoma

作　　者：白松[1] 邵佳发[2] 王维琦[2] 钟孝斌[2] 赵翔宇[2] 

机构地区：[1]昆明医学院第一附属医院干疗科,云南省昆明市650032 [2]昆明医学院,云南省昆明市650032

出　　处：《世界华人消化杂志》2011年第15期1585-1590,共6页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;No.30271667

摘　　要：目的:检测人大肠腺癌标本中hARD1的表达及其与肿瘤分化的相关性.方法:收集昆明医学院第一附属医院手术切除的大肠癌患者大肠腺癌组织、癌旁组织及大肠正常切缘组织标本100例,排除术前化疗的2例,共98例,其中高分化腺癌30例,中分化腺癌27例,低分化腺癌31例,黏液腺癌10例.细胞免疫化学法检测hARD1在大肠腺癌组织、大肠腺瘤和正常大肠组织中的表达.应用图像分析技术检测hARD1在不同大肠癌组织中的表达差异.结果:hARD1在人大肠腺癌组织中高表达(78.57%),大肠腺瘤组织中低表达(33.33%),正常大肠组织几乎不表达(0%).hARD1在人高分化腺癌中的表达明显高于中、低分化腺癌(P<0.05),中分化腺癌中的表达又高于低分化腺癌(P<0.05),且在黏液腺癌中表达更低(P<0.05).结论:hARD1在大肠癌组织、大肠腺瘤和正常大肠组织表达有差异.hARD1在不同分化的大肠癌组织中表达也有差异.其中在高分化腺癌中表达最高.AIM：To examine the expression of human arrest defective 1（hARD1）in human colorectal carcinoma and to analyze its relationship with tumor differentiation.METHODS：A total of 98 colorectal carcinoma samples（including 30 cases of well-differentiated adenocarcinoma,27 cases of moderately differentiated adenocarcinoma,31 cases of lowly differentiated adenocarcinoma,and 10 cases of mucous adenoma）were collected from the First Affiliated Hospital of Kunming medical College and used in this study.Immunohistochemistry was used to detect hARD1 expression in the above tissue samples.RESULTS：hARD1 was highly expressed in colorectal carcinoma（78.57%）,moderately or lowly in adenoma（33.33%）,and hardly found in normal colorectal tissue（0%）.hARD1 expression was significantly higher in well differentiated adenocarcinoma than in moderately and lowly differentiated adenocarcinoma（P〈0.05）,in moderately differentiated adenocarcinoma than in lowly differentiated adenocarcinoma（P〈 0.05）,and in adenocarcinoma than in mucous adenoma（P〈0.05）.CONCLUSION：hARD1 expression was high in colorectal carcinoma,and differential expression of hARD1 was detected among colorectal carcinoma,adenoma and normal colorectal tissue.hARD1 expression was related to tumor differentiation in colorectal adenocarcinoma.

关 键 词：大肠腺癌 hARD1 细胞免疫化学 分化 

分 类 号：R735.34[医药卫生—肿瘤]