早期脑损伤新生大鼠脑白质神经蛋白聚糖和多功能蛋白聚糖表达及意义  

Expression and significance of whife matter neurocan and versican in the newborn rats with brain damage

作　　者：覃海茂[1] 姜志梅[2,3] 郭岚敏[2,3] 张虎[4] 

机构地区：[1]佳木斯大学研究生学院,黑龙江佳木斯154003 [2]佳木斯大学附属第三医院发育与行为儿科 [3]佳木斯大学儿童神经康复实验室,黑龙江佳木斯154003 [4]佳木斯大学基础医学院遗传教研室

出　　处：《中国中西医结合儿科学》2011年第3期216-219,共4页Chinese Pediatrics of Integrated Traditional and Western Medicine

摘　　要：目的观察宫内感染致脑损伤仔鼠脑白质Neurocan和Versican表达情况。方法 40只受孕母鼠随机分为对照组(n=10)和脂多糖组(n=30),给予受孕18 d的脂多糖组孕鼠脂多糖(血清型055)380μg/kg,连续2 d腹腔注射;对照组孕鼠腹腔注射同等剂量的生理盐水。每组随机选取幼鼠各60只,于0,1,7,14,21,28日龄各处死10只仔鼠,HE染色法检测脑组织病理变化,荧光定量RT-PCR方法测定Neurocan和Versican相对表达量。结果对照组孕鼠无死亡及提前分娩,共娩出活产足月仔鼠124只;脂多糖组中孕鼠5只死亡,6只提前分娩,剩余19只共娩出活产足月仔鼠132只,死亡24只。与对照组比较,0,1,7,14,21,28日龄脂多糖组新生大鼠脑白质Neurocan mRNA和Versican mRNA表达增加,差异有统计学意义(P<0.05)。结论宫内感染致脑损伤后,脑白质区Neurocan和Versican的表达明显上调。Objective To investigate Neurocan and Versican expression in white matter of the newborn rats with brain damage caused by intrauterine infection.Method Forty pregnant female rats were randomly divided into control group（n=10） and LPS group（n=30）.The rats in LPS group were intraperitoneally administered LPS（serotype 055） 450μg/kg in two consecutive days,and the rats in control group were intraperitoneally administered physiological saline in the same dose.The offsprings were killed respectively at 0 day,1 day,1 week,2 weeks,3 weeks and 4 weeks after birth;pathological changes of brain tissue were observed by HE staining,and the relative content of the neurocan and versican were measured by fluorescence quantitative RT-PCR.Results（1）There were no death of pregnant rats and early birth in control group,and parturition of live births and full-term pups were 124;5 pregnant rats in LPS group were dead,6 early delivery,and 132 were delivered full-term and live births,death 24.（2）Neurocan mRNA and versican mRNA expression in white matter of LPS group were increased significantly at 0 day,1 day,1 week,2 weeks,3 weeks and 4 weeks,respectively（P0.05）.Conclusion Neurocan and versican expression are increased significantly after brain injury induced by intrauterine infection.

关 键 词：脑损伤 神经组织蛋白类 脑室周围组织软化 脑性疾病 大鼠 新生 动物 实验 

分 类 号：R-33[医药卫生]