5-Aza-CdR对人食管癌细胞株中T-cadherin基因表达和细胞增殖的影响  

Effect of 5-Aza-CdR on Expression of T-cadherin Gene and Cell Proliferation in Human Esophageal Carcinoma Cell Line

作　　者：景钊[1] 龚玲[2] 

机构地区：[1]温州医学院附属第一医院放化疗科,325000 [2]温州医学院附属第一医院感染科,325000

出　　处：《胃肠病学》2011年第5期290-292,共3页Chinese Journal of Gastroenterology

基　　金：温州市科技计划项目(No.Y20090054)资助

摘　　要：背景:T-cadherin在肿瘤的发生中可能扮演肿瘤抑制基因的角色,在食管癌等多种肿瘤中的表达明显下降。目的:探讨去甲基化制剂5.氮杂.2'.脱氧胞苷(5.Aza.CdR)对人食管癌细胞株EC109中T-cadherin基因表达和细胞增殖的影响。方法:常规培养人食管癌细胞株EC109,并分为5μmol/L.5.Aza.CdR组和对照组。以甲基化特异性PCR(MSP)法检测T-cadherin基因启动子区甲基化状态,RT.PCR和蛋白质印迹法分别检测T-cadherin mRNA和蛋白表达.MTT法检测EC109细胞增殖。结果:对照组EC109细胞中T-cadherin基因启动子区呈异常甲基化状态,5-Aza-CdR干预可逆转甲基化状态。与对照组相比.5.Aza.CdR组T-cadherin基因mRNA和蛋白表达均显著增高(P<0.01),细胞增殖明显受到抑制。结论:去甲基化制剂5.Aza.CdR通过逆转食管癌细胞中T.cadherin基因启动子区甲基化状态来增强其表达,并抑制肿瘤细胞增殖。Background： T-cadherin may function as a tumor suppressor gene in the process of tumorigenesis, and down- regulation of T-cadherin in various human cancers has been reported, including esophageal carcinoma. Aims： To investigate the effect of demethylating agent 5-azacytidine-2＇-deoxycytidines （5-Aza-CdR） on expression of T-cadherin gene and cell proliferation in human esophageal carcinoma cell line ECI09. Methods： Human esophageal carcinoma cell line EC109 was cultured conventionally and divided into 5 Ixmol/L 5-Aza-CdR treatment group and control group. Methylation-specific PCR （MS]？） was used to detect the methylation of T-cadherin gene promoter. RT-PCR and Western blotting were used to assess expressions of T-cadherin mRNA and protein, respectively. Proliferation of EC109 cells was measured by MTY assay. Results： T-cadherin gene promoter was hypermethylated in control EC109 cells, and the methylation was reversed after treatment with 5-Aza-CdR. Expressions of T-cadherin mRNA and protein were significantly increased （P〈0.01） and cell proliferation was obviously inhibited in 5-Aza-CdR treatment group than in control group. Conclusions： Demethylating agent 5-Aza-CdR can effectively increase the expression of T-cadherin by reversing the promoter hypermethylation in human esophageal carcinoma cells, and inhibit the cell proliferation.

关 键 词：5-氮杂-2’-脱氧胞苷 钙黏着糖蛋白类 食管肿瘤 甲基化 

分 类 号：R735.1[医药卫生—肿瘤]