胱抑素C和非浓缩尿蛋白电泳联合检测在诊断肿瘤患者化疗中早期肾损伤的应用  

Application of combined detection of cystatin C and non-concentrated urine protein electrophoresis in the diagnosis of early renal injury in patients with cancer and treated with chemotherapy

作　　者：肖大平[1] 史惠强[1] 邵洁[1] 戚前明[1] 

机构地区：[1]中国人民解放军第三五九医院检验病理科,江苏镇江212001

出　　处：《国际检验医学杂志》2011年第8期847-848,850,共3页International Journal of Laboratory Medicine

摘　　要：目的探讨血清胱抑素C(Cystatin C)和尿蛋白电泳联合检测在诊断肿瘤患者化疗中早期肾损伤的临床应用价值。方法健康对照组36例和肿瘤化疗组82例进行Cystatin C的免疫透射比浊法检测,并采用十二烷基硫酸钠-琼脂糖凝胶电泳法(SDS-AGE)对其新鲜晨尿进行尿蛋白电泳检测。结果肿瘤化疗组的Cystatin C含量显著高于健康对照组(P<0.01),肿瘤化疗组的Cystatin C检测阳性率为47.6%,尿蛋白电泳检测阳性率为58.5%,两者联合检测阳性率可达68.3%。结论血清Cys-tatin C和尿蛋白电泳联合检测,可提高化疗患者早期肾功能损害的诊断敏感性和特异性,并有助于判断肾脏损害部位,对肾损害的早期诊断有较高价值。Objective To investigate the clinical application of combined detection of serum cystatin C and urine protein electrophoresis in the diagnosis of early renal injury in patients with cancer and treated with chemotherapy. Methods 36 healthy individuals （healthy group） and 82 cases of patients with cancer and treated with chemotherapy （experimental group） were enrolled. Immune turbidimetric assay and sodium dodecyl sulphate-agarose gel electrophoresis （SDS-AGE） were performed to detect cystatin C and urine protein respectively. Results The level of cystatin C in experimental group was significant higher than healthy group （P 〈（0.01）. The positive rate of cystatin C, urine protein electrophoresis and combined detection in experimental group were 47.6%, 58. S% and 68.3% respectively. Conclusion Combined detection of serum cystatin C and urine protein electrophoresis could im prove the diagnostic sensitivity and specificity of early renal injury in patients treated with chemotherapy and could be used for the judgment of lesioin location,indicating application value of it on the early diagnosis of renal damage.

关 键 词：血清 蛋白尿 电泳 胱抑素C 

分 类 号：R730.53[医药卫生—肿瘤]