解毒化瘀颗粒对急性肝衰竭小鼠肝脏TNFRⅠ与TNFRⅡ表达的影响  

Effects of Jiedu Huayu Particles on Expression of TNFR Ⅰ and TNFR Ⅱ in Acute Liver Failure Mice

作　　者：毛德文[1] 陈月桥[1] 韦艾凌[1] 唐农[1] 邱华[1] 张荣臻[1] 

机构地区：[1]广西中医学院第一附属医院肝病治疗中心,广西南宁530023

出　　处：《辽宁中医药大学学报》2011年第7期5-7,共3页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：国家中医药管理局"慢性重型肝炎解毒化瘀"重点研究室资助项目;广西高校百名中青年学科带头人计划资助项目(桂教人:20056409);广西科学研究与技术开发计划应用基础研究专项资助项目(桂科基:0639053)

摘　　要：目的:观察解毒化瘀颗粒对急性肝衰竭小鼠肝脏TNFRⅠ与TNFRⅡ表达的影响,探讨其拮抗肝衰竭的作用机制。方法:采用D-氨基半乳糖联合内毒素腹腔注射法构建小鼠急性肝衰竭动物模型,并分别予解毒化瘀颗粒、乳果糖、安宫牛黄丸干预治疗,HE染色观察肝脏病理改变,TUNEL法检查肝细胞凋亡率,免疫组化检测小鼠肝脏TNFRⅠ与TNFRⅡ的表达。结果:解毒化瘀颗粒干预组肝组织凋亡、坏死程度轻于模型对照组,免疫组化检测的肝脏TNFRⅠ表达量少于模型对照组(P<0.05),TNFRⅡ表达多于模型对照组(P<0.05)。结论:解毒化瘀颗粒可通过下调肝组织TNFRⅠ的表达、上调TNFRⅡ的表达途径抑制肝细胞凋亡,延缓肝脏坏死的进展。Objective ： To observe the particle Jiedu Huayu liver of mice with acute liver failure and TNFR Ⅰ and TNFR Ⅱ expression, and to explore the role of the antagonistic mechanisms of liver failure. Methods ： D-galaetosamine intraperitoneal injection of the joint construction of endotoxin in acute liver failure animal model in mice, and were given Jiedu Huayu particles, laetulose, Angong Niuhuang intervention, HE staining of liver pathological changes, TUNEL method Check the rates of hepatocyte apoptosis, liver immunohistochemistry TNFR I and TNFR II expression. Results ： The intervention group Jiedu Huayu particles liver apoptosis, necrosis in model control group, the liver detected by immunohistochemistry the expression of TNFR I less than model group （P〈0.05）, TNFR Ⅱ expression than the model control group （P〈0.05） .Conclusion ： The particles can be reduced Jiedu Huayu TNFR I expression in liver tissue ,increased expression of TNFR Ⅱ inhibition of liver cell apoptosis pathway, delaying the progress of liver necrosis.

关 键 词：解毒化瘀颗粒 细胞凋亡 TNFRⅠ TNFRⅡ 

分 类 号：R575.3[医药卫生—消化系统]