电针对大鼠局灶性脑缺血再灌注后海马内HSP70表达的影响  

Effects of Electro-acupuncture on HSP70mRNA Expression in Hippocampi of the Rat after Focal Cerebral Ischemia-reperfusion

作　　者：田国伟[1] 张小卿[1] 

机构地区：[1]辽宁中医药大学,辽宁沈阳110032

出　　处：《辽宁中医药大学学报》2011年第7期68-70,共3页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：辽宁省教育厅重点实验室资助项目(2008S144)

摘　　要：目的:探讨电针刺激"人中"穴、双侧"中冲"穴及"风府"穴对大鼠局灶性脑缺血再灌注后海马内热休克蛋白mRNA(Heat Shock Protein,HSPmRNA)表达的影响及意义。方法:采用大鼠大脑中动脉阻断法制备局灶性脑缺血/再灌注模型,然后通过RT—PCR技术,观察大鼠局灶性脑缺血再灌注后海马内HSP70mRNA的表达以及电针刺激对其表达的干预作用。结果:电针刺激能有效的诱导缺血侧海马内HSP70mRNA的表达,增强脑组织对缺血缺氧的耐受能力。结论:电针刺激能够减轻脑缺血再灌注所造成的损伤与其能够诱导HSP70表达增强有关。Objective ： To observe the influence and significance of HSP expression in the brain of the focal cerebral ischemia-reperfusion rat, which is stimulated by electro-acupuncture through ＂REN ZHONG＂, ＂FENG FU＂ and two sides ＂ZHONG CHONG＂ . Methods ： RT-PCR was used to detect the expression of HSPT0mRNA in hippocampi and effect of electro-acupuncture on HSP7OmRNA expression in the brain of the focal cerebral ischemia-reperfusion rat by MCAo. Results ： Electro-acupuncture can effectively induce the expression of HSP70mRNA in hippocampi,and enforce the tolerance of brain in anoxemic situation. Conclusion ： The increase of HSP70mRNA expression is possibly one of the mechanisms of electro-acupuncture protecting cerebral ischemia-reperfusion damage.

关 键 词：电针 HSP70mR-NA 局灶性脑缺血 再灌注 

分 类 号：R-33[医药卫生]