血小板源性生长因子介导的Akt/c-myc信号转导途径在矽肺纤维化形成中的作用  

Expression of AKT and c-myc mediated by PDGF in rats with silicosis

作　　者：罗玲[1] 杨奕[2] 徐洪[2] 魏中秋[3] 张丽娟[1] 程华[1] 袁瑗[1] 李淑钰[1] 杨方[1] 

机构地区：[1]河北联合大学医学实验研究中心,河北唐山063000 [2]河北联合大学教务处,河北唐山063000 [3]河北联合大学基础医学院病理学教研室,河北唐山063000

出　　处：《华北煤炭医学院学报》2011年第3期297-299,共3页Journal of North China Coal Medical College

基　　金：河北省科技支撑计划项目(编号:09276198D);唐山市卫生临床技术研究计划(编号10150204A-25)

摘　　要：①目的探讨血小板源性生长因子(PDGF)AKT通路在矽肺纤维化形成中的作用。②方法选用非暴露式气管灌注法复制大鼠矽肺模型,将其分为对照组(4周组和8周组)、矽肺模型组(4周组和8周组),采用免疫组织化学法检测磷酸化AKT、c-myc蛋白的定位与分布情况,免疫印迹法检测肺组织内PDGF、AKT、磷酸化AKT、c-myc以及I型和III型胶原蛋白的表达。③结果与相应的对照组相比,矽肺模型组大鼠肺组织内PDGF、磷酸化AKT、c-myc蛋白以及I型和III型胶原蛋白的表达均增加(P<0.05);各组间比较AKT蛋白表达无明显改变。④结论 PDGF介导的AKT及其c-myc促进细胞增殖途径,促进了肺组织间质细胞的增生及其胶原的合成,促进了矽肺纤维化的形成与进展。Objective To investigate whether activity of AKT pathway mediated by PDGF could play a promotion role in silicosis.Methods We use the method disposable endobrochial SiO2 powder douche to make the rat model with silicosis.Groups of experimental animals are as follows： control of silicotic model（control 4 and 8 weeks）,silicotic model（control 4 and 8 weeks）.Expressions of PDGF,phospho-AKT and AKT,c-myc and type I and type III collagen were detected by immunohistochemistry and western blot.Results Compared with the control group,expressions of PDGF,phosphor-AKT,c-myc and type I and type III collagen in silicotic model were all increased significantly（P0.05）.While the expressions of AKT have no significant difference among each groups.Conclusion The activity of AKT/c-myc pathway mediated by PDGF possibly induces pulmonary mesenchymal proliferation and collagen synthesis in rats,which promotes forming and development of silicosis.

关 键 词：血小板源性生长因子 矽肺 AKT C-MYC 

分 类 号：R365[医药卫生—病理学]