应用组织芯片技术检测卵巢癌组织中基质金属蛋白酶-2和p185^(HER-2/neu)的表达及其临床意义  

Clinical significances of matrix metalloproteinase-2,and p185^(HER-2/neu) expressions in ovarian cancer by tissue microarray

作　　者：游牧[1,2] 刘文[1] 饶圣宏[3] 徐振山[4] 宋礼华[4] 

机构地区：[1]安徽医科大学生物化学与分子生物学教研室,安徽合肥230032 [2]淮南联合大学医学系,安徽淮南232001 [3]安徽大学生命科学学院,安徽合肥230039 [4]安徽安科生物工程(集团)股份有限公司,安徽合肥230088

出　　处：《蚌埠医学院学报》2011年第5期448-451,共4页Journal of Bengbu Medical College

基　　金：国家863计划资助项目(2004AA215860);安徽省教育厅自然科学研究资助项目(KJ2011B155);淮南联合大学校级科研项目(CYB1002)

摘　　要：目的:研究基质金属蛋白酶-2(matrix metalloproteinase-2,MMP-2)和p185HER-2/neu在卵巢癌组织中的表达及临床意义,探讨MMP-2和p185HER-2/neu联合和单一检测对卵巢癌诊断的价值。方法:利用组织芯片技术结合免疫组织化学SP法检测80例卵巢癌组织及30例正常卵巢组织MMP-2和p185HER-2/neu的表达。结果:MMP-2和p185HER-2/neu在卵巢癌中的阳性率均明显高于正常卵巢组织(P<0.01),MMP-2和p185HER-2/neu的阳性率与分化程度、临床分期及淋巴结转移的差异均有统计学意义(P<0.05~P<0.01),而与组织学类型无明显关系。联合MMP-2和p185HER-2/neu诊断卵巢癌的灵敏度、准确性有所上升,阴性似然比下降。结论:MMP-2和p185HER-2/neu在卵巢癌组织中的表达对卵巢癌的生长、浸润及转移起重要作用,与卵巢癌的分化、临床分期及淋巴结转移相关。Objective：To investigate the relationship of matrix metalloproteinase-2（MMP-2） and p185HER-2/neu protein expressions in the development of ovarian cancer and to assess the value of MMP-2 and p185HER-2/neu for diagnosing and differentiating.Methods：The expressions of MMP-2 and p185HER-2/neu protein of 80 cases of ovarian cancer and 30 cases of normal tissues were examined by immunohistochemistry on tissue microarrays.Results：In ovarian cancer tissue and normal tissue,the positive expression rates of MMP-2 and p185HER-2/neu were significant differences between the two groups（P0.01）.The expressions of MMP-2 and p185HER-2/neu were closely correlated with histological grade,FIGO stage,lymph node（P0.05-P0.01）,but no association was observed with the clinical stage.There was a significant positive correlation between the expressions of MMP-2 and p185HER-2/neu in ovarian cancer（P0.01）.Combined detection of MMP-2 and p185HER-2/neu,the sensitivity and accuracy were increased and negative likelihood ratio was decreased.Conclusions：MMP-2 and p185HER-2/neu expression might strengthen the invasion and metastasis of ovarian cancer.MMP-2 may associate with regulation of p185HER-2/neu.Joint detection of MMP-2 and p185HER-2/neu will increase the specificity of immunoassay,accuracy and expected positivity.They may be valueable indicators in detection of ovarian cancer.

关 键 词：卵巢肿瘤 免疫组织化学 基质金属蛋白酶-2 p185HER-2/neu 组织芯片 

分 类 号：R737.31[医药卫生—肿瘤]