小麦抗条锈病基因Yr5分子标记检测  

Detection of Molecular Marker Linking to Wheat Stripe Rust-resistance Gene Yr5

作　　者：张胜利[1] 周岩[1] 赵俊杰[1] 茹振钢[1] 

机构地区：[1]河南科技学院/河南省高等学校作物分子育种重点学科开放实验室,河南新乡453003

出　　处：《湖北农业科学》2011年第9期1901-1903,共3页Hubei Agricultural Sciences

基　　金：河南省教育厅自然科学基础研究项目(2009B180007);河南科技学院高层次人才引进项目(7004)

摘　　要：为给小麦新品系的抗性遗传基础鉴定及持久抗性新品种选育提供技术支撑,以22个小麦条锈病抗性品种、2个感病品种及12个野生近缘物种为材料,采用PCR方法探讨了小麦条锈病的抗性基因分子标记Yr5-156在这些材料中的分布。结果表明,在多数抗病材料中能扩增出抗病基因Yr5分子标记特异性条带;但在2个感病材料中也扩增出来了同样的特异性条带。表明抗条锈病基因Yr5的分子标记Yr5-156不能作为分子标记辅助育种手段直接应用,需对其做进一步的验证。In order to offer technical support for identification of hereditary basis of resistance in new wheat strains and breeding new persistent resistance varieties, 22 resistance, 2 susceptible and 12 closely related wild species were used as experimental materials. The molecular marker （Yr5-156） linking to wheat stripe rust-resistance gene Yr5 in these species was amplified by PCR. The results showed that specific bands of Yr5-156 were amplified from most resistance materials as well as the two susceptible materials, which indicated that Yr5-156 could not be directly applied for marker-assisted breeding, and need to be further verificated.

关 键 词：小麦 抗条锈病基因Yr5 分子标记 

分 类 号：S512.1[农业科学—作物学]