水稻T-DNA插入启动子捕获系的初步筛选  

Preliminary Screening of Rice Promoter Trap Lines with T-DNA Tagging

作　　者：胡昌泉[1,2] 刘华清[2] 李素一[2] 潘大仁[1] 王锋[2] 

机构地区：[1]福建农林大学生命科学学院,福建福州350002 [2]福建省农业科学院生物技术研究所福建省农业遗传工程重点实验室,福建福州350003

出　　处：《福建农业学报》2011年第2期143-147,共5页Fujian Journal of Agricultural Sciences

基　　金：福建省科技计划--省属公益类科研院所基本科研专项(2009R10035-3);福建省自然科学基金项目(2010J01099)

摘　　要：利用农杆菌介导法将含报道基因β-glucuronidase(GUS)无启动子的转化载体pCAMBIA1300GUSA-Hyg导入籼稻明恢86获得水稻启动子捕获系。水稻启动子捕获系潮酶素抗感分离比χ2分析表明:39个水稻启动子捕获系中有28个为单T-DNA位点插入。2 653个水稻启动子捕获系报告基因GUS表达检测结果表明:83个水稻启动子捕获系在其不同时期、不同器官(包括根、茎、叶、花药、颖壳、胚、胚乳等组织)中检测到GUS表达。其中GUS活性在胚乳有表达的水稻启动子捕获系24个,占检测总数0.91%;GUS活性在花药中有表达的水稻启动子捕获系为19个,占检测总数0.72%。Binary vector pCAMBIA1300GUSA-Hyg containing β-glucuronidase（GUS） without the promoter was introduced into Oryza sativa L.Minghui 86 by agrobacterium-mediated transformation.The χ2 tests on the hygromycin resistant ratio revealed that 28 of the 39 rice promoter trap lines contained a loci T-DNA tagging.GUS histochemical assay at different stages in different organs,such as root,stem,leave,anther,hull,embryo and emdospern,of 2 653 rice promoter trap lines was performed.The results showed that 83 rice promoter lines were GUS positive.Of all tested,24 lines were expressed in endosperm（i.e.,0.91%） and 19 lines expressed in anther（i.e.,0.72%）.Information collected on the rice promoter trap lines could be useful in identifying new rice genes.

关 键 词：水稻 启动子捕获系 T-DNA标签 

分 类 号：Q78[生物学—分子生物学]