APE1、VEGF在非小细胞肺癌组织中的表达及其与预后的关系  

APE1 and VEGF expression in non-small cell lung cancer and its relationship with prognosis

作　　者：沈春燕[1,2] 穆海玉[2] 王忱[3] 多健[3] 佟仲生[3] 

机构地区：[1]天津医科大学研究生院,天津300070 [2]武警医学院附属医院肿瘤科,天津300162 [3]天津医科大学肿瘤医院天津市肿瘤防治重点实验室,天津300060

出　　处：《天津医科大学学报》2011年第2期166-169,193,共5页Journal of Tianjin Medical University

基　　金：天津市应用基础及前沿技术研究计划项目(08JCYBJC05500)

摘　　要：目的:探讨非小细胞肺癌(NSCLC)组织中脱嘌呤/脱嘧啶核酸内切酶1(APE1/ref-1)和血管内皮生长因子(VEGF)的表达特点及其与预后的关系。方法:采用免疫组化法检测78例NSCLC组织中APE1、VEGF的表达情况,血管内皮特异标记物CD34标记并计数肿瘤微血管密度(MVD),分析APE1、VEGF与MVD之间的关系,并结合患者的临床病理特征进行生存分析。结果:APE1、VEGF在NSCLC中的高表达率分别为78.2%和64.1%。APE1表达与吸烟、淋巴结转移密切相关,VEGF表达则与肿瘤大小、分化程度和淋巴结转移密切相关,MVD值与肿瘤大小和淋巴结转移密切相关(P<0.05)。APE1表达与VEGF表达及MVD计数分别呈正相关(P<0.05)。APE1、VEGF表达与患者生存期密切相关(P<0.01)。多因素分析显示,APE1表达是影响NSCLC患者生存率的独立危险因素。结论:NSCLC组织中APE1表达与VEGF表达存在相关性,APE1过表达可能促进肿瘤血管生成,并与NSCLC患者的预后相关。Objective： To evaluate the prognostic value of Apurinic/Apyrimidinic Endonuclease 1（APE1） in patients with non-small cell lung cancer（NSCLC）,and study the features of APE1 expression and its correlation with vascular endothelial growth factor（VEGF） expression.Methods： Expression of APE1 and VEGF was detected by immuno-histochemical technique.MVD was evaluated by immuno-histochemistry with anti-human CD34 antibody.The relationship between APE1,VEGF and MVD was analyzed,and the results with relevant clinical and pathologic data was compared in advance.Results： Positive rate of APE1 and VEGF in NSCLC was 78.2% and 64.1%.The positive rates of APE1 with that of VEGF and also MVD showed significant correlations（P0.05）.APE1 and VEGF expression were related with survival significantly（P0.01）.Multivariate analysis by using COX regression model showed that APE1 was the important independent prognosis factor of survival.Conclusion： APE1 may promote the angiogenesis by regulation the expression of VEGF,and it may be closely related to tumor growth,metastasis and indicates poor prognosis in NSCLC.

关 键 词：非小细胞肺癌 脱嘌呤/脱嘧啶核酸内切酶1 血管内皮生长因子 微血管密度 预后 

分 类 号：R734.2[医药卫生—肿瘤]