螺旋链霉菌的推理选育  

Reasonable Selection for Streptomyces Spiramyceticus

作　　者：邹娟[1] 叶蕊芳[1] 郑黎[1] 吴阳[2] 张立[2] 戴维[2] 

机构地区：[1]华东理工大学生物反应器工程国家重点实验室,上海200237 [2]河南天方药业股份有限公司,河南驻马店463003

出　　处：《化学与生物工程》2011年第5期54-58,共5页Chemistry & Bioengineering

摘　　要：以螺旋链霉菌25-1为出发菌株,分别采用紫外线(UV)、Co60射线、甲基磺酸乙酯(EMS)、氯化锂(LiCl)、UV+LiCl、亚硝基胍(NTG)+UV进行诱变筛选,比较各方法的致死率、正变率、最大提高幅度及对传代稳定性的影响,结果发现UV、Co60射线、UV+LiCl 3种方法对螺旋链霉菌的诱变效果较好,化学诱变剂对螺旋链霉菌的稳定性影响较大;同时采用丙二酸钠和乙酰胺进行高通量筛选,丙二酸钠不但筛选效果好,且对效价有一定的促进作用,乙酰胺对菌株稳定性有一定的影响。With Streptomyces spiramyceticus 25-1 as primitive strain,ultraviolet（UV）,Co60-ray,ethyl methane sulphonate（EMS）,lithium chloride（LiCl）,UV＋LiCl,nitrosoguanidine（NTG）＋UV were used for mutation screening.By comparing the death rate,positive rate,maximum improve percentage and the stability,UV,Co60-ray and UV＋LiCl exhibited better mutation effect on Streptomyces spiramyceticus,and chemical mutagenesis was not good for the stability.In addition,malonate sodium and acetamide were used for high-throughput screening.The result showed that,malonate sodium could not only screen the strains well,but also promote the titer to a certain degree,while acetamide exerted some influence on the stability of the strains.

关 键 词：螺旋链霉菌 诱变筛选 丙二酸钠 乙酰胺 

分 类 号：TQ465.2[化学工程—制药化工] R978.15[医药卫生—药品]