大孔吸附树脂分离纯化普那霉素的研究  

Study on Isolation and Purification of Pristinamycin by Macroporous Adsorptive Resin

作　　者：张雪霞[1,2,3] 李宁[1,2,3] 李晓露[1,2,3] 王健[1,2,3] 王海燕[1,2,3] 林毅[1,2,3] 王秀捧[1,2,3] 蒋沁[1,2,3] 

机构地区：[1]微生物药物国家工程研究中心 [2]河北省工业微生物代谢工程技术研究中心 [3]华北制药集团新药研究开发有限责任公司,河北石家庄050015

出　　处：《化学与生物工程》2011年第6期40-42,共3页Chemistry & Bioengineering

基　　金：国家"重大新药创制"科技专项(2010ZX09401-403)

摘　　要：用大孔吸附树脂法代替溶媒萃取法分离纯化普那霉素。将普那霉素发酵液预处理后,以大孔吸附树脂XAD1600作吸附剂,在吸附流速为2 BV.h-1的条件下进行吸附;再用40%乙醇-60%酸水(含0.1%的乙酸,下同)进行洗脱,去除色素和杂质;最后用90%乙醇-10%酸水以0.5 BV.h-1流速进行洗脱。得到的普那霉素含量达98.5%以上、收率达到64%左右,产品质量符合法国药典规定。该方法操作简便、成本低、收率高,适于工业化生产。The pristinamycin was isolated and purified by macroporous adsorptive resin instead of by solvent extraction method.The experiment showed that pristinamycin content and yield reached above 98.5%,about 64%,respectively,through adsorption by XAD1600 as absorbent at a flow speed of 2 BV·h-1,and desorption by 40% ethanol-60% H2O containing 0.1% CH3COOH to remove the coloured impurities and minor components firstly,desorption by 90% ethanol-10% H2O containing 0.1% CH3COOH at a flow speed of 0.5 BV·h-1 finally.The quality of the product conformed to the requirements of the French pharmacopoeia.This method fits for industrial production with simple operation,low cost and high yield.

关 键 词：普那霉素 大孔吸附树脂 吸附 分离纯化 

分 类 号：TQ460.72[医药卫生—药物分析学]