纤维素酶水解玉米秸秆的影响因素研究  

Research on the Influential Factors of Corn Stalk's Hydrolysis by Cellulase

作　　者：王娜娜[1] 杨翔华[1] 张全[2] 姚秀清[1] 

机构地区：[1]辽宁石油化工大学环境与生物工程学院,辽宁抚顺113001 [2]抚顺石油化工研究院,辽宁抚顺113001

出　　处：《化学与生物工程》2011年第6期80-82,共3页Chemistry & Bioengineering

摘　　要：考察了预处理方式、纤维素酶加量、初始葡萄糖含量等因素对玉米秸秆酶解效果的影响。结果表明,经过稀酸和蒸汽爆破预处理玉米秸秆的酶解得率分别为97.0%和91.0%,均高于未经预处理玉米秸秆;对蒸汽爆破玉米秸秆,纤维素酶加量与酶液的滤纸酶活不成正比关系,纤维素酶加量越多,平均葡萄糖生成速率(GR)越大;葡萄糖对纤维素酶水解具有反馈抑制作用,随着初始葡萄糖含量的增大,GR呈递减趋势。为优化利用玉米秸秆为原料生产燃料乙醇的工艺提供了依据。The effects of pretreatment mode,cellulase amount and initial glucose content on corn stalk′s hydrolysis by cellulase were studied.The results showed that the enzymolysis yields for pretreated corn stalks were higher than that of untreated one,and the yields of the two kinds of corn stalk which had been pretreated by methods of dilute acid and steam-explosion were 97.0% and 91.0%,respectively.The steam-explosion pretreated corn stalk′s was hydrolyzed by several cellulases,which resulted that there was no proportion relationship between the cellulase amount and filter paper activity（FPIU） of cellulase solution.Additionally,more cellulase was added,more average glucose generated rate（GR） was achieved.At the same time,GR was decreased with the increase of initial glucose content which demonstrated that glucose had the feed back inhibition to the hydrolysis of cellulose.This study provided a basis for optimization of production technology of fuel ethanol with corn stalk as material.

关 键 词：纤维素酶 玉米秸秆 水解 预处理 

分 类 号：TQ353.6[化学工程] Q556[生物学—生物化学]