槲皮素对结肠癌细胞SW480体外侵袭的影响  

Inhibitory Effect of Quercetin on Invasion of Colon Cancer Cell SW480

作　　者：孟勇[1] 李华[2] 林增海[1] 吴华涛[1] 

机构地区：[1]汕头大学医学院第一附属医院普外科,广东省汕头市515041 [2]西安交通大学第二附属医院普外科,陕西省西安市710000

出　　处：《中华全科医学》2011年第7期999-1000,1003,共3页Chinese Journal of General Practice

基　　金：2009年广东省中医药局科研课题(2009232);2009年广东省科技攻关项目(2009B030801321)

摘　　要：目的观察槲皮素处理后人结肠癌SW480细胞细胞形态和结构的变化;观察槲皮素对体外培养的人结肠癌SW480细胞的生长的作用;观测槲皮素对细胞体外侵袭、黏附和运动能力的影响。方法应用光学显微镜和电子显微镜观察细胞形态结构的变化;观测不同剂量和时间情况下槲皮素对SW480细胞的作用。采用人工重组基底膜技术观察药物对细胞侵袭、运动和黏附的影响。结果经槲皮素处理后SW480细胞的数量减少。部分细胞的体积缩小,形状不规则,细胞浆空泡形成,细胞核浓缩,染色质凝集。透射电镜观察SW480细胞超微结构的变化显示:内质网高度肿胀,核糖体减少,染色质边集,细胞核固缩,还可见凋亡小体。槲皮素处理SW480细胞后细胞数量减少并呈时间和剂量依赖性。槲皮素处理后SW480细胞的侵袭、运动及黏附能力下降,并呈时间和剂量依赖性。结论槲皮素对人结肠癌SW480细胞的生长有抑制作用,并能诱导其凋亡;槲皮素抑制SW480细胞的侵袭、运动和黏附,是在多个层面抑制肿瘤的侵袭和转移。Objective To investigate the appearance and structure changes of SW 480 after treated by quercetin,and to explore the effects of Quercetin on invasion,metastasis and adhesion in human SW480 cell.Methods The morphological changers of SW480 were investigated by microscopically and electro microscopically examination.The effect of different concentrations of Quercetin on SW480 cell for 24,48,72 hour was observed;Matrigel Basement Membrane Matrix was used to investigate the effects of Quercetin on invasion,metastasis and adhesion in human SW480 cell.Results Light microscopic observation showed that the amount of SW480 cells decreased,partial cells volume shrunk,the morphology of SW480 was irregular,cytoplasmic condensation,vacant bubbles,condensed nuclei and condensed chromatin after treated with Quercetin.Under electron microscope,SW480 cell exhibited that endoplasmic reticulum swollen,ribosome decreased,cytoplasmic condensation,pyknotic nuclei and apoptotic bodies;The amount of human colon carcinoma cell line SW480 treated by Quercetin decreased with dose and time dependent.After treated by Quercetin,the ability of invasion,metastasis and adhesion descended with dose and time dependent.Conclusion Quercetin can inhibit the growth of human colon carcinoma cell line SW480 by inhibiting the ability of invasion,metastasis and adhesion and induce cell apoptosis.Many key points in the invasive and metastasis process were enrolled in.

关 键 词：槲皮素 结肠癌 侵袭 转移 

分 类 号：R735.35[医药卫生—肿瘤] R730.3[医药卫生—临床医学]