百里醌对人骨肉瘤SaOS-2细胞生长的抑制作用  

Effects of thymoquinone on growth and apoptosis of osteosarcoma SaOS-2 cell line in vitro

作　　者：沈为栋[1] 尚雷[2] 刘岸[1] 沈跃[3] 廖维[3] 胡丽萍[4] 黄莉莉[4] 

机构地区：[1]中南大学湘雅医学院岳阳临床学院,湖南岳阳414000 [2]中南大学湘雅二医院 [3]温州医学院附属第二医院 [4]温州医学院

出　　处：《中华实验外科杂志》2011年第7期1054-1056,共3页Chinese Journal of Experimental Surgery

摘　　要：目的探讨百里醌对体外人骨肉瘤SaOS-2细胞生长的影响及其机制。方法百里醌作用人骨肉瘤SaOS-2细胞24h后，细胞计数CCK-8法检测细胞增殖；流式细胞仪检测细胞凋亡;DAPI染色后荧光显微镜下观察细胞形态学变化；Western blot检测SaOS-2细胞中Caspase-3、X连锁凋亡抑制蛋白（XIAP）和Smac的表达。结果不同浓度（20、40、80μmol／L）百里醌作用人骨肉瘤SaOS-2细胞24h后，细胞存活率分别为（75．5±4．2）％、（62．1±6．7）％和（52．5±4．9）％；细胞早期凋亡率分别为（8．1±0．7）％、（13．2±1．1）％和（20．2±1．7）％；百里醌作用后，SaOS-2细胞出现典型凋亡的形态学改变；百里醌可明显上调Caspase-3和Smac在SaOS-2细胞中的表达，而显著抑制XIAP的表达。结论百里醌具有抑制体外人骨肉瘤SaOS-2细胞生长和促进细胞凋亡的作用，可能是通过上调人骨肉瘤SaOS-2细胞中Caspase-3和Smac的表达和下调XIAP的表达而实现。Objective To investigate the inhibitory effect of thymoquinone on the growth of human osteosarcoma cell line SaOS-2 in vitro. Methods After human osteosarcoma SaOS-2 ceils were treated with different concentrations of thymoquinone, the proliferation was measured by Cell Counting Kit-8 （ CCK-8 ） assay. The flowcytometry （FCM） was used to determine apoptosis of SaOS-2 cells. The morphological changes of SaOS-2 cells were observed under the fluorescence microscopy after DAPI staining. Western blotting was used to detect the protein expression of Caspase-3, X-linked inhibitor of apoptosis protein （ XIAP） and Smac. Results Thymoquinone obviously suppressed proliferation of SaOS-2 cells in a dose-dependent manner, and the apoptosis rate was （ 8.1 ± 0. 7 ） % , （ 13.2 ± 1.1 ） % and （ 20. 2 ±1.7 ） % respectively when the concentrations of thymoquinone exposed were 20, 40 and 80 μmol/L. After treatment with thymoquinone, the expression of Caspase-3 and Smac was up-regulated in SaOS-2 cells, and thymoquinone treatment significantly decreased the XIAP levels in SaOS-2 cells. Conclusion Thymoquinone has the anti-tumor effect on the human osteosarcoma SaOS-2 cells in vitroprobably by up-regulating the expression of Caspase-3 and Smac and down-regulating the expression of XIAP.

关 键 词：百里醌 骨肉瘤 脱噬作用 

分 类 号：R738.1[医药卫生—肿瘤]