1α,25-(OH)_2D_3对体外培养成骨细胞内钙离子浓度的影响  

EFFECT OF 1α,25-(OH)_2D_3 ON [CA^(2+)]_i OF OSTEOBLASTS CULTURED IN VITRO

作　　者：顾建红[1] 蒋杉杉[1] 刘俊栋[1] 熊桂林[1] 闫汶[1] 刘宗平[1] 

机构地区：[1]扬州大学兽医学院,扬州225009

出　　处：《营养学报》2011年第3期243-246,共4页Acta Nutrimenta Sinica

基　　金：国家自然科学基金(No.30972229,30571364);江苏省自然科学基金(No.BK2008214,BK2009736);校创新培育基金(No.2010CXJ059)

摘　　要：目的研究不同浓度1α,25-(OH)2D3(0、10-11、10-9、10-7 mol/L)对体外培养成骨细胞(osteoblasts,OB)内游离钙离子浓度([Ca2+]i)的影响及钙离子通道机制。方法在体外培养SD大鼠OB基础上,添加不同浓度的1α,25-(OH)2D3或/和10-8 mol/L硝苯地平(NIF)作用20 min,流式细胞仪测定[Ca2+]i。结果 10-11 mol/L 1α,25-(OH)2D3组[Ca2+]i显著低于对照组(P<0.05),10-9、10-7 mol/L组[Ca2+]i显著或极显著高于对照组(P<0.05或P<0.01);单独添加10-8 mol/L NIF组[Ca2+]i与对照组差异不显著(P>0.05);联合添加10-9 mol/L 1α,25-(OH)2D3和10-8 mol/L NIF组[Ca2+]i与对照组差异不显著(P>0.05),但显著低于单独添加10-9 mol/L 1α,25-(OH)2D3组(P<0.05)。结论 1α,25-(OH)2D3引起[Ca2+]i改变的过程涉及L-型钙离子通道。Objective To study the effect of 1α,25-（OH）2D3 on [Ca2＋]i of osteoblasts cultured in vitro and its mechanism.Method Osteoblasts isolated from calvaria bone of SD rats were treated with various concentrations of 1α,25-（OH）2D3 and nifedipine（NIF） 20 min later,and the i of osteoblasts was evaluated.Results The [Ca2＋]i in the group with 10-11 mol/L 1α,25-（OH）2D3 was lower than that in the control group significantly（P0.05）.Compared with the control group,the [Ca2＋]i in the group with 10-9 mol/L and 10-7 mol/L 1α,25-（OH）2D3 increased significantly or very significantly（P0.05 or P0.01）,but was not changed in NIF group alone or 1α,25-（OH）2D3 plus NIF group.However,the [Ca2＋]i in the combined group（10-9 mol/L 1α,25-（OH）2D3 plus 10-8 mol/L NIF） was lower than that in the group with 10-9 mol/L 1α,25-（OH）2D3 significantly（P0.05）.Conclusion L-type calcium channel plays an important role in the change of [Ca2＋]i in osteoblasts induced by 1α,25-（OH）2D3.

关 键 词：成骨细胞 1α 25-(OH)2D3 硝苯地平 钙离子 

分 类 号：R151[医药卫生—营养与食品卫生学]