脂肪酶和青霉素酰化酶催化合成手性化合物的研究进展  

Solvent effect on catalytic synthesis of chiral compouds with lipase and penicillin G acylase

作　　者：薛屏[1] 曹雪荣[1] 

机构地区：[1]宁夏大学能源化重点实验室,宁夏银川750021

出　　处：《化工进展》2011年第7期1577-1583,共7页Chemical Industry and Engineering Progress

基　　金：国家973计划前期研究专项(2010CB534916);国家自然科学基金(21064005)项目

摘　　要：生物酶催化合成手性药物和中间体具有高效、节能和环境友好等突出的优势,本文从提高催化反应效率的角度,评述了非水相酶催化反应的溶剂效应——溶剂体系对酶的活性和对映选择性的调控作用;介绍了动力学控制合成β-内酰胺类抗生素的不同溶剂体系,包括有机物-水共溶剂体系、有机物-水不共溶剂体系、反胶束体系和双水相体系。提出利用溶剂效应调控动力学合成体系,会有效提高酶催化合成β-内酰胺类抗生素的产率和合成与水解比S/H值,从而实现底物的有效利用。Synthesis of chiral drugs and intermediates catalyzed by enzyme has high efficiency,energy saving and environment-friendliness advantages.This paper reviews the solvent effect on the enzymatic reaction in non-aqueous media with lipases,namely,the activity and enantioselectivity of enzyme adjusted by solvent system to improve the efficiency of catalytic reaction.The kinetically controlled synthesis of β-lactam antibiotics with penicillin G acylase was introduced in different solvent systems,including the organic-water miscible solvents,organic-water immiscible solvents,reverse micelles and aqueous two-phase system.The yield of kinetically controlled synthesis of β-lactam antibiotics and synthesis/hydrolysis ratio（S/H）could be enhanced by designing an effective solvent system to better utilize the substrates.

关 键 词：溶剂效应 脂肪酶 青霉素G酰化酶 手性化合物 对映选择性 

分 类 号：O643.4[理学—物理化学]