银纳米颗粒对神经元的毒性研究  

Toxicity of Silver Nanoparticles to the Neurons

作　　者：周国凤[1,2] 汤京龙[2] 熊玲 周亮[1] 许建霞[2] 邵安良[1,4] 杨丽娜[2] 陈艳梅[2] 李雪飞[2] 奚廷斐[1,2,4] 

机构地区：[1]温州医学院,浙江温州325035 [2]中国药品生物制品检定所,北京100050 [3]成都市食品与药品检测中心,成都610041 [4]北京大学深圳研究院,广东深圳510087

出　　处：《北京生物医学工程》2011年第3期301-305,共5页Beijing Biomedical Engineering

基　　金：国家自然科学基金(30770579);973项目(2007C13936101);863项目(2007AA021901);中国药品生物制品检定所中青年研究发展基金(2009C4)资助

摘　　要：目的研究不同浓度的银纳米颗粒对神经元毒性的剂量-效应关系,探索银纳米颗粒对神经元的毒性机理。方法首先培养一种活性好、生长状态优良的原代神经元,将不同浓度(2.5～500μg/mL)的银纳米颗粒加入神经元中作用24h后,通过MTT法计算细胞增殖率,并分析不同浓度的银纳米颗粒对神经元毒性的剂量-效应关系。结果在25～250μg/mL范围内,细胞数量与形态呈现不同的变化,银纳米颗粒浓度与细胞增殖率呈负相关。结论在25～250μg/mL的浓度范围内,银纳米颗粒对神经元细胞的毒性呈剂量-效应关系。Objective To study the dose-effect relationship between silver nanoparticles with different concentrations and the neurotoxicity, and also explore the mechanism of silver nanoparticles to neurotoxicity. Method A group of primary neurons in excellent growth status was cultured, and then contacted with different concentrations （2.5 -500μg/ml ）of silver nanoparticles for 24h. After that we calculated the cell proliferation rate by MTT test and analyzed the dose-effect relationship between the concentrations of silver nanoparticle with neurotoxicity. Result Within the silver nanoparticle concenrtation of 25 -250μg/ml, both cellular mount and morphology changed, and it was showed anticorrelation between silver nanoparticle concentration and cell proliferation rate. Conclusion Within the sliver nanoparticles concenrtation of 25 -250μg/ml, silver nanoparticles shows a dose-effect relationship on neurotoxicity.

关 键 词：银纳米颗粒 神经元 毒性 

分 类 号：R318.08[医药卫生—生物医学工程]