桑枝总黄酮的抗氧化活性研究  

Antioxidation properties of total flavones from Mori Ramulus

作　　者：章丹丹[1] 高月红[1] Jessica Tao Li 潘一峰 卞卡[1,3] 

机构地区：[1]上海中医药大学穆拉德中药现代化研究中心,上海201203 [2]上海现代中医药技术发展有限公司,上海200051 [3]美国德克萨斯大学休斯顿医学院综合生物及药理学系德克萨斯大学分子医学研究所,休斯顿tx77030

出　　处：《中成药》2011年第6期943-946,共4页Chinese Traditional Patent Medicine

基　　金：国家科技重大专项重大新药创制(2009ZX09311-003);国家自然科学青年基金(81001666);国家科技部十一五支撑计划(2006BAI11B08-03);上海市教委高校一氧化氮与炎症医学E研究院计划(E-04010);上海市科委国际技术转移专项(08430711300);上海市科委中药现代化专项(08DZ1972104);上海高校选拔培养优秀青年教师基金资助项目(SZY07029)

摘　　要：目的研究桑枝总黄酮于无细胞系统中的直接抗氧化活性和干预细胞后的体外抗氧化活性。方法桑枝总黄酮经提取和D101大孔树脂纯化后获得,并用紫外分光光度法测定。采用DPPH法、ABTS法和FRAP法分析桑枝总黄酮的直接抗氧化活性且用trolox做阳性对照;采用ABTS法、FRAP法和Griess法分别测定桑枝总黄酮干预AngII诱导内皮细胞模型和LPS协同IFN-γ诱导巨噬细胞模型中的抗氧化活性。结果桑枝总黄酮于100μg/mL剂量对ABTS自由基清除能力>对DPPH自由基清除能力>对铁离子的还原能力;在AngII诱导内皮细胞ECV304模型中显示清除ABTS自由基的能力,且强于对铁离子的还原能力;同时能显著降低LPS协同IFN-γ诱导巨噬细胞中亚硝酸盐的量。结论桑枝总黄酮于体外直接测定或干预细胞后均显示具有抗氧化活性。AIM The antioxidant properties of total flavones from Mori Ramulus（TFMR） were analyzed in vitro with either extracted total flavones or total flavones treated cells.METHODS Total flavones were extracted and purified from Mori Ramulus with D101 macroporous resin.Concentration of total flavones was quantified by Spectrophotometer.DPPH assay,ABTS assay and FRAP assay were employed to analyze the antioxidant activity of total flavonoids with trolox as control.The antioxidant effect of TFMR on AngII-treated endothelial cells and LPS/IFN-γ-treated macrophages were evaluated with ABTS/FRAP assay and Griess reaction respectively.RESULTS TFMR（100 μg/mL） exhibited a significant antioxidant capacity in the order of ABTSDPPHFe3＋ reduction.TFMR showed higher potency in scavenging ABTS radical compared with reducing Fe3＋ in AngII-treated ECV304 cells.TFMR markedly decreased nitrite accumulation in macrophages stimulated with LPS/IFN-γ.CONCLUSION The antioxidant properties of TFMR were demonstrated in vitro by directly measuring total flavones or total flavones treated cells.

关 键 词：桑枝总黄酮 抗氧化活性 FRAP法 DPPH法 ABTS法 Griess法 

分 类 号：R287[医药卫生—中药学]