丙酮介质中辣椒素酯的酶催化合成动力学研究  

Study on the Kinetics of Lipase-Catalyzed Synthesis of Capsinoids in Acetone Media

作　　者：刘绍[1,2] 刘卉琳[1] 董新荣[3] 谢达平[1] 

机构地区：[1]湖南农业大学生物科技学院,湖南长沙410128 [2]湖南农业大学食品科技学院,湖南长沙410128 [3]湖南农业大学理学院,湖南长沙410128

出　　处：《分子催化》2011年第3期276-280,共5页Journal of Molecular Catalysis(China)

基　　金：湖南省自然科学基金项目(07JJ6026)

摘　　要：对脂肪酶在丙酮介质中催化香草醇和壬酸甲酯合成辣椒素酯的反应动力学进行了研究.以HPLC方法测定酶催化合成产物辣椒素酯的浓度,确定了反应初速度;采用双倒数作图分析法和产物抑制试验法研究了本合成反应的机制,结果表明辣椒素酯的酶催化合成是按乒乓反应机制进行的.研究还确定了酶反应动力学方程和Vmax、KmA、KmB的值,并对其进行了验证,结果表明动力学方程与试验结果拟合度达0.997.The reaction kinetics for synthesis of capsinoids by lipase-catalyzed transesterification of vanillyl alcohol and methyl nonanoate in acetone was investigated in this work.The concentration of capsinoids obtained in the reaction was detected by HPLC and then the initial velocity of the synthesis reaction was calculated.The inhibiting test of product was carried out with methanol added.The results revealed that the enzymatic-synthesis reaction follows the Ping-Pang reaction mechanism.The kinetic equation with corresponding Vmax,KmA and KmB was inferred by the double reciprocal plot.The fitting degree of equation model arrives at 0.997 to compare with the experimental data.

关 键 词：辣椒素酯 酶催化反应 脂肪酶 有机介质 动力学 

分 类 号：Q555.3[生物学—生物化学] O643.32[理学—物理化学]