中国人细小病毒B19系统发育关系研究  

Phylogenetic study of human parvovirus B19 distributed in China based on bayesian inference and maximum likelihood analysis

作　　者：何苗[1] 高蕾[1] 柯玲[1] 刘鱼[1] 曾沛斌[1] 李武平[1] 

机构地区：[1]中国医学科学院北京协和医学院输血研究所,四川成都610052

出　　处：《中国输血杂志》2011年第5期399-402,共4页Chinese Journal of Blood Transfusion

摘　　要：目的探讨在中国分布的人细小病毒B19的系统发育。方法对B19病毒阳性的23份来自中国献血者和5份B19/HIV共感染者标本,采用病毒基因组NS1-VP1-u区测序后,与Genbank下载的参考序列比对,用软件MRBAYES 3.1.2作贝叶斯推断,用在线软件RaxML作最大似然法运算,绘制进化树并分析。结果 28份来自中国的标本均属于B19-1A亚型,且呈现复系,其中来自新疆乌鲁木齐来自四川的各1例标本与非中国序列聚在一起,其他中国26份标本与来自美国的病毒株Au聚在一枝并有着很好的支持率,来自西安的2条参考序列出现在B19-1A亚型的基部。结论中国的B19病毒是多起源的,其在中国中西部地区进化速率较慢;应该积极开展不同地区B19病毒多样性的研究。Objective To perform phylogenetic analysis of Human Parvovirus B19 distributed in China based on Bayesian Inference and Maximum Likelihood methods.Methods Segments of NS1-VP1-u region from 28 B19 DNA positive samples were amplified by nested PCR.After alignment,MRBAYES 3.1.2 was used for Bayesian Inference analysis and RaxML web-server was employed to construct Maximum Likelihood tree.Results Our samples in this study which appeared to be polyphyletic should be placed in B19-1A subgenotype.One sample from Urumqi and one sample from Sichuan clustered with the sequences from foreign countries,respectively,while the other 26 samples solidly clustered with strain Au from USA.Two reference sequences from Xi′an presented at the basal place of group B19-1A.Conclusion B19 virus should be multiregional origin with relatively slow substitution rate in central and western China.Further study is awaited to investigate the diversity of Human Parvovirus B19 in large-scale area in China.

关 键 词：人细小病毒B19 系统发育 贝叶斯推断 最大似然法 

分 类 号：R373[医药卫生—病原生物学] R457.13[医药卫生—基础医学]